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TRIM33 deletion in Trim33fl/fl Cre-ERT2 mice significantly impaired DC differentiation from hematopoietic progenitors at different developmental stages. TRIM33 deficiency downregulated the expression of multiple genes associated with DC differentiation in these progenitors. TRIM33 promoted the transcription ...
Invaded cells were stained with crystal violet and the absorbance was measured at 560 nm. (c) Representative images of colony formation assay. (d) Quantification of colonies formed. The colony formation efficiency was significantly reduced in both SUB1 siRNA1- and 2-treated cells as compared ...
and that the mRNA abundance of bothHdac2andHdac3increased at 1 and 3 days post ONC [10]. Here, we used fluorescence microscopy to monitor the expression of HDAC3 protein in cells of the GCL ofHdac3fl/flcompared toRosa26-LacZfl/flmice, 5 days post ONC. Fluorescence microscopy images...
TRPC3 knock-down in MDG cells; MG29, mitsugumin-29; ROCE, receptor-operated Ca2+-entry; RyR, ryanodine receptor; SOCE, store-operated Ca2+-entry; SR, sarcoplasmic reticulum; STIM, stromal interaction molecule; TRPC, canonical-type transient receptor potential cation channel Abstract During membran...
Here, we show that RECQ1 is highly expressed in various types of solid tumors. However, only in the case of brain gliomas, the high expression of RECQ1 in glioblastoma tissues is paralleled by a lower expression in the control samples due to the poor expression of RECQ1 in non-dividing ...
USA). Then, after transfection for 24, 48 and 72 h, 100μl Cell Counting Kit-8 (CCK-8) (Dojindo, Kumamoto, Japan) solution was added into each well and the plates were incubated in a incubator for 1 h. The absorbance was measured at a wavelength of 450 nm using a microplate reader...
Surprisingly, we observed 2- and 14-fold reductions in all three HIV-1 mRNA forms with 100 nM and 1 μM sudemycin D6, respectively (Fig. 3A). To confirm that inhibition of SF3B1 abrogates the production of all HIV splice forms, we used Western blots to probe for HIV proteins ...
In general, early apoptosis is harmful to virus replication, thus p53 competition is a core factor for both the host and virus in the infection process [23]. Metal ions such as calcium (Ca), iron (Fe), manganese (Mn), and zinc (Zn) are crucial for life [24]. Deficiencies in Fe ...
Measurement of the FITC-dextran concentration was carried out on a Cary Eclipse fluorescence spectrophotometer (Agilent, Santa Clara, CA, USA; excitation, 490 nm, emission, 520 nm). Emission signals in mice treated with 40 kDa dextran were excluded from blank values (33% normal mouse plasma in...