Murine tissues were harvested from KrasLSL − G12D, p53flox/flox mice27–29. IHC was performed following resection from mice, fixation in formalin and embedding in paraffin. Flow cytometry analysis of integrin expression was performed using the following antibodies: integrin α5 (Abcam and ...
(ES) cells were electroporated with R26TV-LSL-TRL and five clones were generated, one of which was positive forRosa26integration by PCR screening and Southern analysis (Fig. 2f,Supplementary Fig. S7). These targeted ES cells report Cre recombination via both luciferase (Fig. 2g,Supplementary ...
Following in vitro validation, C57BL/6J-Tyr c−2J embryonic stem (ES) cells were electroporated with R26TV-LSL-TRL and five clones were generated, one of which was positive for Rosa26 integration by PCR screening and Southern analy- sis (Fig. 2f, Supplementary Fig. S7). These ...
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