Our previous studies determined that IncHI2 plasmids are lcianskeesdhtaovethbeeednistdroibcuutmioenntoefdo.qFxlAuoBr-obqlauCinTXo-lMongeesnseus cinh E. as coli and Salmonella spp.10,11. However, only a few of t
pA is used to transform a strain of DH10B with the genomic copy of auxG knocked out via recombineering, which is grown and made competent in medium containing the auxotrophic compound (Aux) to maintain cell viability. Following outgrowth in medium containing Aux (mimicking excluding the ...
Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid’s ability to persist in a population. However, for a community consisting of multiple populations tran
These findings indicate that borrelias organize their total cellular DNA into several complete genomes and that cells undergoing serotype switches do one or more of the following: (1) coexpress Vmps from switched and unswitched expression plasmids for at least three to five generations, (2) ...
An equilibrium is stable if the real parts of both eigenvalues of the following Jacobian matrix evaluated at this equilibrium are negative: sp−(α+βr)Nr,p−2αNF,p−(α+βr)NF,p(βr−α)Nr,psp−c−2αNr,p+(βr−α)NF,p Three cases depending on the value of βr ...
Immediately following elec- troporation, cells were suspended in 500 μl of SOB, and an appropriate aliquot was spotted on an SOB agar plate containing 2 μg/ml imipenem for selection of blaKPC-positive E. coli transformants. Detection of carbapenemase activity. Activity of class A/B...
If there are gene dosage effects, smax would increase with n; we do not consider this in our numerical examples, but all results for a given n also apply to this scenario. We have assumed above that every cell has n copies of the plasmid of interest. To maintain this number, we assume...
Answer to: Indicate whether each of the following statements is true or false. Animal cells have small, circular pieces of DNA in the cytoplasm...
How is messenger RNA processed before leaving the eukaryotic cell? Describe how the DNA code is translated into messenger RNA What is a segmented RNA virus? Is EBV an RNA virus? Is ribosomal RNA made of nucleotides? Which of the following describe plasmids, circular double stranded RNA, circula...
The following plasmids with fusions of this type are present in four Eurasian isolates: lp36/lp28–3 fusion in B. garinii Far04 that has an "orphan" lp28–3 PFam32 gene (not in a partition cluster), lp28–3/lp28–4/ lp38 fusion in B. valaisiana VS116 (at this point only the ...