Three broad host-range MLS plasmids, pAMβ1 (Em), pIP501 (Em, Cm) and pJH4 (Em, Sm, Km), were transferred by a conjugation-like process from Streptococcus faecalis to Clostridium acetobutylicum. The plasmids were stably maintained and antibiotic resistances were fully expressed in C. ...
Three broad host-range MLS plasmids, pAMβ1 (Em), pIP501 (Em, Cm) and pJH4 (Em, Sm, Km), were transferred by a conjugation-like process from Streptococcus faecalis to Clostridium acetobutylicum. The plasmids were stably maintained and antibiotic resistances were fully expressed in C. acetobut...
Horizontal gene transfer (primarily conjugation) could explain this persistence, but it has been suggested that very high conjugation rates would be required. Here, we show that common conjugal plasmids, even when costly, are indeed transferred at sufficiently high rates to be maintained in the ...
We determined the antimicrobial susceptibilities and resistance genes of mcr - 1 -positive isolates and investigated the transferability of polymyxin resistance by plasmid conjugation. Pulsed-field gel electrophoresis, plasmid profiles, and Southern blot were used to analyze genetic relationships and plasmid...
Transconjugant 6L2 was the second colony on the filter mating plate from parental EntC-6 strain at low concentration of rifampin in the conjugation experiment. The blaACT-like genes were revealed by PCR and DNA sequencing. The blaCMH-1 was confirmed by PCR, cloning and DNA sequencing. The ...
Conjugation experiments were performed to show resistant marker transfer. The most prevalent isolates Escherichia coli (E. coli) 58.6%, Klebsiella Spp. 32.9% and Pseudomonas 8.6%, were resistant to most of the antimicrobials including cefazolin, ceftriaxone, cefuroxime, ampicillin and co-trimoxazole ...
The genetic organization of the blaCTX-M-15 genes has been elucidated in the two host plasmids obtained by conjugation experiments (Tc20 and Tc319), in which blaCTX-M-15 gene transfer was successful. In addition, these transconjugants were characterized by their resistance genes diversity. ...
pMB2 is self-transmissible by virtue of a complete IncFIIF-type conjugation system. The growth of laboratory strains of E. coli that have reduced growth rates when carrying a 90 Kb plasmid was not affected by carriage of the larger pMB2. Conclusion: Plasmid-borne quinolone resistance genes ...
The MCR-1 was found encoded by a plasmid highly mobilizable by conjugation to the E. coli J53 strain. Two E. coli strains carried two determinants, mcr-1 plus pmrA or pmrB mutations, known to confer colistin resistance. 展开 关键词: Colistin resistance mcr-1 PmrAB Plasmid S. enterica E...
(HGT) via the mobilizable plasmid pVP52-1 from the donorVibrio parahaemolyticusNJIFDCVp52 to the recipientEscherichia coliEC600 by 1.47- to 3.19-fold. The transcription levels of tetracycline resistance genes [tetA,tetR(A)], conjugation-related genes (traA,traD), outer membrane protein genes (...