质粒小提试剂盒(25)英文名称:Plasmid Mini Kit(25)品牌:Qiagen货号:12123规格:25次产品特点:纯化得到相当于2次氯化铯梯度离心得到的DNA纯度重复性好,高产量质粒DNA经济实惠使用LyseBlue,获得*的裂解和*的DNA产量传统的QIAGEN Plasmid Giga Kits应用重力流阴离子交换柱QIAGEN-tip 20 高效纯化质粒DNA,也单独提供QIAGEN-...
This unique method for plasmid preparation in 96-well format provides highly pure, transfection-grade plasmid DNA using a simple bind-wash-elute procedure (see flowchart " QIAGEN PlasmidPlus 96 procedure"). The QIAGEN Plasmid Plus 96 Miniprep protocol starts with a modified alkaline lysis proced...
Plasmid DNA encoding constitutively expressed GFP (pEGFP-C2) was prepared using either Monarch Plasmid Miniprep Kit or Qiagen QIAprep Spin Miniprep Kit. Four different cell lines (Cos-7, HEK293, HeLa, and Huh-7) were grown to 80-90% confluence and transfected with 100 ng of each plasmid, ...
Please see the Troubleshooting Section of theQIAprep Miniprep Handbookand Appendix A of theQIAGEN Plasmid Purification Handbookfor instructions, and a picture and legend explaining the typical results you may see. You can also access this information on ourPlasmid Resource Pages. ...
德国QIAGEN,货号:16181,QIAGEN Plasmid Plus 96 Miniprep Kit (4) 价格电议 起批量≥1 盒 最小起订1盒 供货总量66盒 发货地址广东省深圳市 建议售价￥0/盒 更新日期2024年12月27日 即时洽谈立即询价查看联系方式 收藏产品发送留言 VIP9年 深圳子科生物科技有限公司 ...
品牌:Qiagen 货号:12163 本产品目前正在促销中,产品价格为折后价格,折扣为75折,欢迎来点咨询。 1.公司简介: 上海创赛科学仪器有限公司(Shanghai Canspec Scientific Instruments Co.,Ltd.)(以下简称:上海创赛),是一家致力于实验室全套用品和设备(包括:生物、化学化工、实验耗材、仪器设备等)的集约化供应商。上海...
Start QIAGEN miniprep protocol by adding 180µl of Buffer P1 to obtain a final volume of 250µl Add 250µl Buffer P2 Etc.. follow QIAGEN protocol Elute DNA in 30µl of H2O Use 20µl of eluted DNA to transform 200µl competent XL1-Blues ...
QIAGEN Plasmid Plus 96 Kits提供创新的方法通过96孔板纯化质粒。样本可在BioRobot Universal System上通过QIAvac 96和/或离心机处理或自动运行。使用专有的结合试剂,确保从5 ml E. coli培养液中每孔可纯化获得至多50 μg转染级质粒DNA。独特的内毒素去除缓冲液可使内毒素水平低至1 EU/μg DNA。 产品特点 Features...
QIAGEN PlasmidPlus96 Kits提供创新的方法通过96孔板纯化质粒。样本可在BioRobot Universal System上通过QIAvac 96和/或离心机处理或自动运行。使用专有的结合试剂,确保从5 mlE. coli培养液中每孔可纯化获得至多50 μg转染级质粒DNA。独特的内毒素去除缓冲液可使内毒素水平低至1 EU/μg DNA。
at least 10 single colonies were picked and grown at 37 °C in LB medium supplemented with appropriate antibiotics. The plasmid DNA was purified from the cultures using Qiaprep Spin Miniprep Kit (Qiagen, Germany) following the manufacturer’s protocol. The purified plasmids were then digested ...