常见大肠杆菌种系 更多大肠杆菌信息可参考(https://blog.addgene.org/plasmids-101-common-lab-e-coli-strains?_ga=2.210903381.1761310573.1650342339-1554568351.1648354532) 2. 常用于质粒选择的抗生素 许多质粒被设计成包含抗生素抗性基因,当其表达时,仅允许含有质粒的细菌在含有该抗生素的培养基中或培养基上生长。这些...
1. 了解质粒包含的元件 当今科学家使用的质粒有多种大小,功能差异很大。在最简单的形式中,质粒需要细菌复制起点 (ori)、抗生素抗性基因和至少一个独特的限制酶识别位点。这些元件允许质粒在细菌内繁殖,同时允许对任何不携带质粒的细菌进行选择。此外,限制酶位点允许将待研究的 DNA 片段克隆到质粒中。 Origin of Repli...
产品编号 D101-100 规格: 100 rxns数量 - + 库存: 999 立即购买 加入购物车 询价 查看购物车 产品描述 说明书 相关文献 客户好评 产品简介 柱式质粒 DNA 小量抽提试剂盒是巨匠生物最新研制成功的一种质粒小量抽提试剂盒。本产品采用了一种新型的吸附材料,新吸附柱拥有良好的流速、超强的 DNA 结合...
Plasmid is degraded. SomeE. colistrains (HB101 and the JM series) have high levels of endogenous endonuclease. Avoid using these when possible. If these strains are used, keep samples on ice during prep and ensure the Plasmid Wash Buffer 1 step is performed. ...
we integrated the DNA assembly method and the software with a robotic system named Illinois Biological Foundry for Advanced Biomanufatcuring (iBioFAB) (Supplementary Fig.1) to create a nearly fully automated workflow for PlasmidMaker. As a proof of concept, we constructed 101 plasmids across six...
The minimal replication origin (ori) of the plasmid pSC101 was defined as an about 220-bp region under the condition that the Rep (or RepA) protein, a plasmid-encoded initiator protein, was supplied in trans. The DnaA box is located at one end of ori, as in other plasmids, like mini...
Plasmid pws101, for its preparation and its use Plasmid pws101 characterized in that: & lt; br / & gt; it may be multiplied in the case of microorganisms of the species saccharomyces cerevisiae and of th... W Siede 被引量: 0发表: 1991年 pWS101 plasmid, method of obtaining same and...
电话:400-800-2947 工作QQ:1843439339 (微信同号) 邮件:Biovector@163.com 手机:18901268599 地址:北京 已注册 详细信息 咨询记录(共0条) PSC101 TIMER plasmid vector原核低拷贝组成型荧光表达质粒载体 low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under...
ZPK101-1 50T 200 - + 有货 ZPK101-2 100T 280 - + 有货 ZPK101-3 200T 500 - + 有货 产品介绍 说明书下载 ■ 产品简介 本试剂盒采用碱裂解法裂解抽提细菌质粒。优化后的质粒抽提溶液系统,较传统的质粒提取更快速,更顺畅。离心吸附柱中采用的硅基质材料为本公司特有新型材料,高效、专一吸附DNA。