// 我们统计每个样本中属于B/plasma cell的数量 // 只保留包含100个以上B/plasma cell的样本 retain.cell = length(which(rna.data$ct %in% c("B","plasma cell","memory B","naive B","immature B","preB","proB","proliferation pre/pro B","IgA plasma","IgG plasma","GC B","pro B","pr...
The aims of the study were to assess GPRC5D gene expression and cell surface localization in myeloma cells, the changes in expression of GPRC5D and other typical myeloma cell markers following coculture of primary myeloma cells with osteoclasts and the consequences of GPRC5D gene silencing on ...
Hierarchical cluster analysis with these genes revealed that previously defined MM gene expression subgroups (MM1-MM4) could be linked to one of the 3 normal cell types. Clustering with 30 vEDGs revealed that 13 of 18 MM4 cases clustered with tonsil BCs (P = .000 05), whereas 14 of 15...
Then the corresponding protein-coding gene expressions were also analyzed regarding their causal effect on PC and their expression pattern in single-cell types. The function and involved pathways of these targets were preliminarily investigated through enrichment analysis, knock-out mice models, and ...
cell types differed by race with increased plasma cells in PC-B representing the largest difference (d) (Wilcoxon Rank Sum FDRP-values;n = 300 patients). Of these, increased plasma cell content in PC-B was the only difference in both validation cohorts (Wilcoxon Rank Sum). Gene ...
Molecular cloning and functional expression of a new human CC-chemokine receptor gene. Biochemistry 35, 3362–3367, 10.1021/bi952950g (1996). Article CAS PubMed Google Scholar Duell, E. J. et al. Variation at ABO histo-blood group and FUT loci and diffuse and intestinal gastric cancer ...
Structural and functional analysis of disease-associated mutations in GOT1 gene: an in silico study[J]. Computers in Biology and Medicine, 2021, 136: 104695. DOI: 10.1016/j.compbiomed.2021.104695 [23] Bednarz-Misa I, Fleszar MG, Fortuna P, et al. Altered l-arginine metabolic pathways in...
DNA was extracted using a QIAamp blood kit (Qiagen) and plasma DNA measured, in genome equivalents/milliliter plasma (GE/mL), using real-time quantitative PCR for the β-globin gene. Prior to biopsy, plasma DNA concentration in BPH patients was 936 GE/mL (median; range: 633–2074 GE/...
Cell-free plasma DNA was measured by real-time polymerase chain reaction assay for the β-globin gene and SOFA score, APACHE II score, CRP concentrations, and clinical outcome (duration of stay, ventilation time, and mortality) were noted in 94 patients on admission to the ITU. The median ...
cellsurfaceproteins that identify plasma cells and B cells at different stages of development, it is possible to identify and recover immature and mature plasma cells from various locations[12,14,15]. This allows for functional studies on these cells after isolation, including migration andgene ...