Plasma-cell-enriched gate of a peripheral blood sample demonstrates a prominent population of abnormal plasma cells, which are positive for CD38, CD117 (partial), CD138, and intracellular lambda light chain restriction (dim). The abnormal plasma cells are negative for CD45 and CD56. ● Variable...
Most of the plasma cells detected in the harvests from myeloma patients were immature (CD45+/CD45++) rather than mature (CD45-). When the total plasma cell population was studied, definite isotype restriction could be detected in only 16% of harvests. Light chain restriction was found in ...
albicans cell, whereas the turquoise arrowhead shows GFP-C. albicans signal within a CD45+ leukocyte. c, Representative image of the superior sagittal sinus (SSS) region from the indicated groups, stained for B220 (green) and Ki-67 (red), showing a peri-SSS cluster of Ki-67+B220+ ...
IgD+CD23+mature B cells, and CCR6+memory B cells among total B220+CD19+thymic B cells by flow cytometry (Fig.5A, B). PBs specifically expressed genes associated with cell proliferation (Fig.4B, C), and we detected Ki67+cells among CD138+Blimp1+cells (Fig.5C). Thus, we validated...
Plasma cellsare cytoplasm Ig (cIg+) and express surface CD38. A small subset ofmyelomacells express CD10 and 15–20% expressCD20. CD56 (N-CAM) is expressed on the majority of malignant plasma cells, but CD34 is not. The clonogenic cell inMMhas not been demonstrated with certainty. There ...
Multiple myeloma (MM) cells inhibit certain T-cell functions. We examined the expression of B7-H1 (PD-L1), a B7-related protein that inhibits T-cell respon... J Liu,A Hamrouni,D Wolowiec,... - 《Blood》 被引量: 565发表: 2007年 Plasma cells in Multiple Myeloma express a natural kill...
"Constraining CD45 exclusion at close-contacts provides a mechanism for discriminatory T-cell receptor signalling". "Chapter 24 – Xenopus extract approaches to studying microtubule organization and signaling in cytokinesis". "Elastin-PLGA hybrid electrospun nanofiber scaffolds for salivary epithelial cell ...
Results/Conclusion Consensus guidelines support i) the use of minimum of five initial gating parameters (CD38, CD138, CD45, forward, and sideward light scatter) within the same aliquot for accurate identification of the total plasma cell compartment; ii) the analysis of potentially aberrant ...
Our analysis confirmed the presence of three intratumoral B cell populations, based on CD19, human leukocyte antigen – DR subtype (HLA-DR), CD38, and Ki67 staining (plasma cells: CD38+, HLA-DR−, Ki67−; GC B cells: CD38+, HLA-DR+, Ki67+; follicular B cells: CD38−, HLA-DR...
Cells were sorted into plates, were sequenced and underwent quality control evaluation and filtering out of non-plasma cell contaminants (Online Methods). c, Table summarizing the total CD38+CD138+ cell numbers from bone marrow and blood of newly diagnosed individuals and control donors, that were...