We hypothesized that lack of ATG8-delipidating activity would result in a large number of EosFP–ATG8E ΔC-decorated autophagosomes fusing with the vacuolar membrane, which should eventually yield fluorescent labeling of the tonoplast (Fig. 2F). Indeed, induction of autophagy caused accumulation ...
For M. huakuii-specific probe, fragments amplified with the primer pair RAA-F/RAA-R using genomic DNA of M. huakuii as a template were labeled with Biotin- 14-dUTP (containing CY3 Label) via the Random Primer method of BioPrime DNA Labeling System (Invitrogen™ Thermo Fisher Scientific (...
The PCR products were amplified using Phusion High Fidelity DNA polymerase (NEB) and a pRS300 plasmid containing the miR319a precursor as the template109,110. The engineered artificial microRNAs were subcloned into the pCR8/GW/TOPO vector (Thermo Fisher) and transferred into a Gateway Cloning ...
By using the natural architecture of plant tissue, we could effectively engineer a multiplicity of mammalian tissue depending upon the plant template. Matching native mechanical properties have shown to be a crucial aspect when engineering tissue. Since a wide variety of anatomical structures exist with...
The primer which binds to many different loci is used to amplify random sequences from a complex DNA template that is complementary to it (maybe including a limited number of mismatches). Amplification can take place during the PCR, if two hybridization sites are similar to one another (at ...
Using pCBC-DT1T2 as the template, the sgRNA (PUX4)-U6-26t-U6-29p-sgRNA (PUX5) cassette was amplified by PCR and inserted into pHEE401 by GoldenGate assembly to obtain pHEE401-PUX4/PUX5. The sgRNA (PUX3) was cloned into pHEE401, which was then digested with BsaI to obtain the ...
Small peptides represent a subset of dark matter in plant proteomes. Through differential expression patterns and modes of action, small peptides act as im
The analysis provides two intrinsic model-accuracy estimates (predicted template modelling (pTM) and interface pTM (ipTM)) and we used a combination of these two estimates as the confidence metric. An ipTM + pTM score of 0.5 or more is considered to be indicative of a reliable interaction...
Evidence from a common garden study with five native species. Restor Ecol 2010; 19: 268–276. Article Google Scholar Johnson GR, Sorensen FC, St Clair JB, Cronn RC . Pacific Northwest forest tree seed zones: a template for native plants? Native Plants J 2004; 5: 131–140. Article ...
Promoter molecules can also include DNA sequences from which transcription of transfer RNA (tRNA) or ribosomal RNA (rRNA) sequences are initiated. Transcription involves the synthesis of a RNA chain representing one strand of a DNA duplex which provides the template for its synthesis. Transcription...