Depending on their sizes, stems were aligned in parallel and embedded in 1% agarose in PBS to include multiple biological replicates in the same resin block. Samples were then processed through increasing dehydration steps (25%, 50%, 70%, 90%, 96%, 3× 100% ethanol, vacuum 20 inHg, ...
The polymerized resin block was also used for microCT (SkyScan 1272, Bruker) imaging, and the microCT data were processed using Amira (v.2020.3) software. Lignin content and composition determination Samples were ground, lyophilized and extracted successively with chloroform/methanol (2:1, v/v)...
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The top side of the U-frame is larger (30 mm) that the cover slot in order to block the frame. It bears a hole in its middle, designed to insert an Araponics seed holder (http://www.araponics.com/) and supports the aerial rosette. Rhizoponics blueprints and 3D files are ...
2001). Extracellular chitinases quickly block the spreading of the hyphae that invade internal areas. It also helps to release fungal elicitors that induce the synthesis of several other chitinases inside the host (Stangarlin and Pascholati 2000). Unlike other plant proteins, PR proteins are ...
Dyslipidemia is an independent risk factor of cardiovascular diseases (CVDs), which lead to the high mortality, disability, and medical expenses in the worldwide. Based on the previous researches, the improvement of dyslipidemia could efficiently prevent
(a) Surface view of a glochidic trichome. (b) BSE image of the sectioned block face showing compositional contrast. Higher brightness indicates higher content of the mineral elements Ca and P in the hooks. (c) EDX spectra of the hook tips and the main wall show high calcium phosphate ...
Although initially and extensively studied in the context of apoptosis, caspases are increasingly considered as versatile molecular switch controlling different cellular outcomes. In particular, inhibition of caspase-8 not only fails to block cell death, but also causes inflammatory lytic cell death by ...
Although initially and extensively studied in the context of apoptosis, caspases are increasingly considered as versatile molecular switch controlling different cellular outcomes. In particular, inhibition of caspase-8 not only fails to block cell death, but also causes inflammatory lytic cell death by ...
For example, non-annular lipids act as cofactors to block ion conduction and regulate the activity of membrane proteins. 5.2. Metal Coordination of Lipids with Membrane Proteins In recent years, great progress has been made in the structural analysis of membrane proteins. However, the interactions ...