The William Madison Randall Library, University of North Carolina, Wilmington, NC, holds the public documents for the Brunswick plants. Carolina Power & Light Co., (Operator); Owned by: Progress Energy (81.67%); NC Eastern Municipal Power Agency Cape Fear River Brunswick Steam Electric Plant: ...
In mountainous areas, wild edible plants are an important part of the local diet. Climate change and anthropogenic activities have profound effects on wild edible plants in these areas. Ethnobotanical studies are important for understanding the use patterns and harvest impacts on these plants. In thi...
DNA methylation is an evolutionarily conserved epigenetic mechanism essential for transposon silencing and heterochromatin assembly. In plants, DNA methylation widely occurs in the CG, CHG, and CHH (H = A, C, or T) contexts, with the maintenance of
For pull-down experiments, the PhBSβ CDS was amplified, cloned into the NdeI and HindIII sites of pET32b expression vector in-frame with a C-terminal 6×His-tag (Novagen, Madison, WI, USA) using ClonExpress II One Step Cloning Kit (Vazyme), and the protein was expressed in E. coli....
visualized on agarose gels. Samples with failed amplification were excluded from further analysis. Amplified DNA was purified using the Wizard SV Gel and PCR Clean-Up System (Promega Corporation, Madison, USA) and pooled prior to Illumina amplicon sequencing at Eurofins Genomics (Konstanz, Germany)...
Researchers from University of Wisconsin, Madison, USA have attempted to explain in detail about fluorescent protein (FP)-based biosensors that are being viewed as an unique modes through which dynamic cellular networks can be monitored for transgenic modifications. Green fluorescent protein based biorep...
To determine whether EV proteins can trigger plant responses, we analyzed proteins from purifiedP. sojaeEVs by liquid chromatography/tandem mass spectrometry (LC/MS-MS). We identified 468 EV proteins with more than three unique peptides detected in each of the three repeats (Supplementary Data1)...
Sci. USA, 85:2444 (1988), by computerized implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, Wis.), or by manual alignment and visual inspection. One example of a useful ...
Optimal alignment of sequences for aligning a comparison window may be conducted by algorithms; preferably by computerized implementations of these algorithms (for example, the Wisconsin Genetics Software Package Release 7.0-10.0, Genetics Computer Group, 575 Science Dr., Madison, Wis.). The reference...
Sequence similarity can be determined by comparing the nucleotide sequences of two nucleic acids using sequence analysis software such as the Sequence Analysis Software Package of the Genetics Computer Group, University of Wisconsin Biotechnology Center, Madison, Wis. ...