A PCR screen of the putative GalNAc-T2 transgenic plants was performed with GoTaq DNA Polymerase (Promega, Madison, WI), 1 μg genomic DNA (DNeasy Plant Mini Kit, Qiagen, Valencia, CA) as a template, and the same primers and PCR program used for GalNAc-T2 gene amplification. Total RNA...
Ciliogenesis in these cells occurs de novo, as opposed to the canonical template pathway seen in animal lineages [21]. It can thus be assumed that there are regulatory mechanisms that ensure correct spatial and temporal expression of genes required for ciliary function within this restricted phase ...
deposited in GenBank under accession number AJ970307, was used as a template. At the beginning of the design procedure, all possible 70-mer nucleotides (with a 1-nucleotide shift) were generated for each strand. We analyzed their nucleotide composition, melting ...
4–5 putative transmembrane domains and termini of 60–70 residues (Supplementary Fig. 1). Using phylogenetic analyses, we found that the protein sequence was highly conserved in land
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A PCR screen of the putative GalNAc-T2 transgenic plants was performed with GoTaq DNA Polymerase (Promega, Madison, WI), 1 μg genomic DNA (DNeasy Plant Mini Kit, Qiagen, Valencia, CA) as a template, and the same primers and PCR program used for GalNAc-T2 gene amplification. Total RNA...
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The pTRA-TaARL6IP4 vector was constructed with the UTR and TaArl6ip4-R4b primer sets (Table S1) using the pTRA-TaArl6ip4-GFP vector as a template. The pTRA-TaARL6IP4 vector was then introduced into Agrobacterium tumefaciens strain GV3101 via the electroporation method. Agrobacterium-mediat...
The aim of the work was to study the biological interference of the spontaneous colonization of pathogenic and saprophytic endophytes on the nitrogen assimilation of mycorrhized wheat plants cultivated in soils deficient in N and P. The nitrogen assimila