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Phytomedicine is one of the top options for the treatment of chronic illnesses for millions of people around the world. To learn about the bioactive components of medicinal plants, their medical benefits, and their synergistic or additive effects to enhance the action of medications, substantial new...
a–cJA inhibits the expressions of genes related to secretary vesicles and membrane. Ten-day-oldArabidopsis(Col-0) andaoswere wounded and leaves were harvested 2 h post wounding (W). The untreated plant leaves were used as control. Total RNAs were extracted for RNA sequencing and qRT-PCR ...
, Academic Press, San Diego, Calif. (1990). The mutant PAP-encoding DNA (e.g., a cDNA) is preferably inserted into a plant transformation vector in the form of an expression cassette containing all of the necessary elements for transformation of plant cells. The expression cassette typically...
and DNA delivery systems. For example, types of transient analyses can include but are not limited to direct gene delivery via electroporation or particle bombardment of tissues in any transient plant assay using any plant species of interest. Such transient systems would include but are not limited...
Plant viruses provide naturally occurring nanoparticles that are being repurposed for gene delivery into plant cells. Credit: Nicole Steinmetz/UCSD For this project Giraldo teamed up with Nicole Steinmetz, a UC San Diego professor of nanoengineering, to utilize nanotechnologies engineered by her team th...
The community structure and abundance of the plant microbiota are influenced by the amount of glutamic acid available via exudates or by exogenous delivery. Given that glutamic acid is naturally present in host exudates, it would seem that plants already have the potential to engineer protective micr...
Protoplast-mediated delivery of CRISPR/Cas9 ribonucleoproteins offers advantages for creating non-transgenic mutant plants23,24,25,26,27,28,40. However, regenerating plants from protoplasts can be difficult and has not been demonstrated to be possible for many important crop species. Low efficiency of...
More preferably, expression vectors are introduced into plant tissues by using either microprojectile-mediated delivery with a biolistic device or by using Agrobacterium-mediated transformation. Transformant plants obtained with the protoplasm of the subject basil plants of the present application are ...
Several methods are available for introducing DNA sequences into plant cells and are well known in the art. Suitable methods include but are not limited to bacterial infection (e.g., with Agrobacterium as described above), binary bacterial artificial chromosome constructs, direct delivery of DNA (...