These primed seeds were then placed in PTC jam bottles containing MS media with culturing conditions like those used in PTC (see materials and methods). The use of clarified supernatants in priming significantly accelerated and improved germination responses in wheat seeds under these in vitro ...
After autoclaving, the sterile medium was dispensed into sterile jam bottles. All the cultures were incubated at 22 ± 2 °C under white light (PFD: ~ 52 μmol m−2 s−1) for a photo-period of 16/8 h light/darkness. All experiments were repeated at least three times. ...
doi:10.1007/s11240-020-01881-8Shivani SinghRobert ThangjamG. D. HarishHardeep SinghAnuradha AgrawalSpringer NetherlandsPlant Cell Tissue and Organ Culture
regular checks (as also with ADJ’s tissue culture regimes) were carried out for 4 weeks. During this time, any contamination that could be attributed to a source(s) other than the explants was excluded from the study, while those that (i) appeared after 4 weeks, and (ii) did ...
the seeds were inoculated into agar medium and left to stand for 2 to 3 weeks in a high-temperature room with full lighting. The seeds were transferred into 200 ml conical beakers in order from those started sprouting, and those growing larger were transferred into jam bottles. The leaves ...
For development of roots, the regenerated shoots were shifted to jam bottles containing rooting medium MROM (comprising half strength MS salts, 30 g/l sucrose, 3.0 g/l phytagel, pH 5.8; 250 mg/l cefotaxime and 30 mg/l hygromycin added after autoclaving) and maintained at 27 ± 1°C in ...
PLANT tissue cultureFOOD cropsBANANASEMBRYOSSEEDSAGRICULTURAL intensificationVITAMIN CEnsets (Ensete spp., family Musaceae), are multipurpose plants with great versatility and potential as a food security crop, although they are cultivated only in Ethiopia and few other regions of Africa. Currently due...
The washed seeds were inoculated on ½ strength MS medium in jam bottles and kept in the dark for 5–7 days. After germination, the seedlings were screened for the presence of the transgene. Screening of putative transgenic plants PCR method The putative transgenic seedlings were screened for ...
were intracellularly delivered to A549 and LAMA84 cells in a time-dependent manner in vitro, and the intraperitoneally injected CLEVs accumulated in the tumor tissue 15 min, 1 h, and 24 h after injection in a mouse model of cancer [117]. Mu et al. confirmed that PDEVs isolated from ...