1. Prepare the proper planting hole for trees and shrubs. How deep should the soil be when planting trees and shrubs? When preparing any hole for tree planting, make it two to three times wider than the current root mass but never deeper than the plant was growing in its previous environm...
Milwaukee, WI, USA). Excess dNTPs and unincorporated primers were removed from the PCR product using the Clean-Up Purification Kit (A&A Biotechnology, Gdynia, Poland). Finally, DNA was eluted in 40 μl H2O and the obtained amplicons were sequenced...
Trolox ((±)-6-hydroxy2,5,7,8-tetramethyl-chroman-2-carboxylic acid, 97%) from Aldrich (Milwaukee, WI) was used as a standard to construct the calibration graph. The reaction mixture contained 0.25 mL of plant extract, 1.75 mL of 80% ethanol, and 2 mL of 0.2 mM DPPH solution. In ...