在模式拟南芥中建立了GRO-seq(通过细胞核run-on在体外亲和标记新生RNA)和pNET-seq(分离RNA聚合酶II结合的RNA)的方法(Nature Plants, 2018);利用新生RNA测序方法,揭示了植物在转录水平对高温胁迫的快速响应及胁迫记忆 (JIPB, 2021);在异源六倍体小麦基因组中,利用新生RNA测序技术首次检测到植物增强子的转录(Genome ...
1. SNP微阵列中预测拷贝数变异CNV,DeepCNV 2. RNA-Seq中预测premiRNA,dnnMiRPre 实操内容 1. 复现DeepCNV利用SNP微阵列联合图像分析识别拷贝数变异 复现循环神经网络RNN工具 dnnMiRPre,从RNA-Seq中预测premiRNA 第五天 理论部分 深度学习在识别及疾病表型及生物标志物上的应用 1. 从基因表达数据中识别乳腺癌分...
RNA-seq reveals differentially expressed genes in rice (Oryza sativa) roots during interactions with plant-growth promoting bacteria, Azospirillum brasilense. PLoS One 14: e0217309. Google Scholar Crossref PubMed WorldCat Tian J, Shu J, Xu L, et al. 2023. Genome-wide identification ...
烟草基因研究中心发表的PCMDB数据库,包含六种模式植物(拟南芥、水稻、玉米、大豆、番茄和烟草)的三种不同类型的细胞标记,包括实验验证的标记基因,基于Bulk RNA-seq数据的差异表达标记基因,以及通过scRNA-seq鉴定的特定细胞间的差异表达基因。 PlantPhoneDB: A manually curated pan-plant database of ligand-receptor p...
Typical downstream applicationNorthern blotting, Primer extension, qRT−PCR, RNA-Seq Storage temperature15–25 °C / 59–77 °F Shelf life (from production)24 Month(s) Hazardous materialYes Lysis Buffer RA1 REF 740961 € NucleoSpin RNA Plus, Mini kit for RNA purification with DNA removal colu...
Single-cell RNA sequencing (scRNA-seq) measurements of gene expression show great promise for studying the cellular heterogeneity of rice roots. How precisely annotating cell identity is a major unresolved problem in plant scRNA-seq analysis due to the i
For sequencing, libraries for each sample were prepared using the Illumina TruSeq Nano DNA kit. Subsequently, paired-end reads of 151 base pairs each were generated using an Illumina HiSeq 4000 NGS (Illumina, San Diego, CA, USA). Data resources To mitigate false-positive variants resulting fro...
在该研究中,基因挖掘和功能分析使用了SLAF-seq、BSA分析、重测序、KASP、RNA-seq、qRT-PCR、PCR克隆等测序和分子实验方法 成功分离适应水淹的主效基因CsARN6.1,并验证基因功能,解析了黄瓜耐水淹分子机制。 百迈客云BSA分析工具是一款结合多年BSA分析项目分析经验开发的包含一键式标准化分析和个性化多样性分析集成式分析...
然后,在第3天收集来自MaBEL1过表达和空载体转化的对照样本进行RNA-Seq分析。在MaBEL1过表达和空载体转化的对照系之间,共鉴定出3055个下调和2147个上调的差异表达基因(DEG)。这些DEG主要富集于次生代谢、苯丙素、氨基酸、植物激素信号转导以及氨基和核苷酸糖和蔗糖的代谢的生物合成(图S7)。乙烯和脱落酸香蕉(图3A、B...
The nuclear DNA was sheared with a Covaris S2 ultrasonicator and the library was sequenced (twice) as 2×250 bp on an Illumina MiSeq. Finally, conventional Sanger reads were generated with an ABI 3730xl sequencer using the Big Dye–terminator Cycle Sequencing kit. Recombinant clones (pJET...