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Notice the word supplement–this is not meant to be the bulk of your protein intake in your diet, protein powders are used to supplement (i.e. make up the deficit) the diet overall. Even if you’re not very active, protein powder still may be a great option to incorporate into your ...
ultimum strains recovered activity after 2 days of culture, and the mycelium was collected after 3 days of growth, and other culture conditions were identical to those for P. sojae. For DNA extraction, 0.3–3 g of mycelium was ground into powder with liquid nitrogen and transferred to a...
Phytopathogens often secrete effectors to enhance their infection of plants. In the case of Sclerotinia sclerotiorum, a necrotrophic phytopathogen, a secreted protein named SsPEIE1 (Sclerotinia sclerotiorum Plant Early Immunosuppressive Effector 1) plays
For DNA extraction, 0.3–3 g of mycelium was ground into powder with liquid nitrogen and transferred to a 50-mL centrifuge tube; 5–25 mL of 2% Lysis Buffer 1 were added to the tube and mixed by shaking. The mixture was incubated at 65 °C for 120 min, then centrifuged at...
2. The samples are ground thoroughly into powder with liquid nitrogen in 1.5-mL microcentrifuge tubes with a pestle. 3. To each ground sample, 200 μL ice-cold protein extraction buffer with protease inhibitor cocktail (Roche) is added; the samples are homogenized on ice for 30 min. 4....
A fraction of powder was added with an equal volume of 2× SDS sample buffer (4% SDS, 100 mM Bis-Tris pH 6.8, 10% glycerol, 2% β-mercaptoethanol), and incubated on ice for 15 min, followed by heating for 10 min at 65 °C. After centrifugation at 13 000× g for 5 min, the ...
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Protein extraction and purification The harvested leaves were grinded into fine powder in a RM200 mortar grinder (Retsch, Haan, Germany), precooled with liquid nitrogen. Leaf midribs were removed from the harvested leaves to facilitate grinding, either at harvest or immediately prior to grinding. ...