This is especially an issue given that this is a quantitative method, and because the same amplicons need to be amplified every time copy numbers are to be determined. In-lab gel electrophoresis is an alternative, albeit one with a risk of carryover contamination. In our preliminary experiments...
There is another source of variation among experiments that is often not taken into account when comparing data. Using the example of light again, two experiments may show different phenotypic responses to light intensity (e.g. a strong positive effect vs. no effect, Fig.1a). Where plant biol...
Laser triangulationThese techniques involve shining a laser beam to illuminate the object of interest and a sensor array to capture laser reflection [8]. Due to the low-cost setup, they are widely used in laboratory experiments [29,30]. Paulus et al. [30] used this technique to produce a ...
The analysis has pinpointed significant publications, sources, authors, institutions, and countries contributing to the scientific progress in plant-based crop protection. From the existing in vitro, in vivo and in planta experiments, we identified 91 plant species from 28 families highlighted for ...
(Methods). Plants were then grown for an additional 24 h in the growth chamber before being prepared for imaging by washing the roots in water. The fluorescence from GFP was measured using confocal microscopy (Methods). As observed in the hydroponics experiments, GFP was only observed in ...
Plant chemical defenses are commonly used as proxies of ecological impacts because they are inducible, often easier to measure than reproduction, and important to pathogen success and herbivore feeding preferences and performance [31,32]. However, in designing these kinds of experiments with insect pes...
Results of three independent measurements of light-induced P700 photo-oxidation in respective experiments are shown in the table. OD, optical density. Full size image Comparison between pre-PSI-1 and mature PSI revealed a series of structural alterations with respect to cofactor and protein ...
But, in most cases, full-scale experiments of nuclear power systems require high capital expenditure. An alternate feasible method is to perform the similar experiments on reduced-scale equipment, and the results obtained from these experiments must be extrapolated to full-scale equipment. However, ...
(typical for wall-based systems) were used as greenery. During the experiments, some plant species burned more, less or not at all. The experiments cannot be directly compared to each other due to the different plant quantities, but they provide initial findings. If a plant was actively ...
Simultaneous labeling of different organelles with specific Tag/Catcher combinations will enable simultaneous isolation of different organelles from one plant extract in future experiments.Background An elegant and innovative way to covalently link proteins and create artificial multiprotein assemblies or team...