Biotic homogenization is primarily driven by native species’ extirpation and the introduction and spread of non-native species, commonly due to human activity11,12,13. The balance and influence of these contributing factors remains largely untested across different scales. For thousands of years, ...
(3) natural commodity: plants that are naturally harvested with little cultivation; (4) feral: plants that are not economically important but have still been influenced by human selection; (5) wild: plants that occur in the wild and have not been directly impacted by humans; and (6) wild ...
However, the mechanisms underlying the pro-proliferative effects of many PDNVs remain unknown. It is important to note that the impact of PDNVs on cell proliferation is influenced by the source of nanovesicles [56]. Cabbage-derived nanovesicles were found to promote the proliferation of human ...
The science of “molecular farming”, or the use of plants and plant cell cultures to produce high-value recombinant proteins, started with the production via transgenic tobacco and sunflower of chimaeric human growth hormone in 1986 [1], then of monoclonal antibodies in transgenic tobacco in 198...
Plant metabolites play a vital role in plant adaptation, in human nutrition and medicine. The diversification of plant metabolic enzymes and primary metabolism evolves potential compounds for cellular homeostasis in selection pressure that is usually supposed to be conserved over the plant kingdom. The...
Kim SM, Suh JP, Lee CK, Le JH, Kim YG, Jena KK (2014) QTL mapping and development of candidate gene-derived DNA markers associated with seedling cold tolerance in rice (Oryza sativa L.). Mol Genet Genom 289:333–343 Article CAS Google Scholar Konishi S, Izawa T, Lin SY, Ebana ...
DNA was extracted using the Qiagen DNeasy kit. For O. secunda, 206 individuals were sequenced for the chloroplast trnS-trnG intergenic spacer, 154 individuals were sequenced for the nuclear internal transcribed spacer (ITS) region, and 218 individuals genotyped for five nuclear microsatellite loci. ...
DNA for SNP genotyping was extracted for each accession from bulked leaf samples of eight 2-weeks old seedlings. A customized oligonucleotide pool assay (IPK-OPA, unpubl) containing 1536 allele specific oligos was used to genotype the panel by Illumina's GoldenGate technology (Illumina, San Diego...
RNA silencing is a central regulator of gene expression in most eukaryotes and acts both at the transcriptional level through DNA methylation and at the post-transcriptional level through direct mRNA interference mediated by small RNAs. In plants and invertebrates, the same pathways also function direc...
we usedAgrobacterium-mediated DNA delivery inNicotiana benthamianaas a transient gene expression platform. This allowed for production of3and4as substrates and the combinatorial testing of selected gene candidates (Methods). We were unable to identify an oxidase that could act directly on4, so we in...