Plant Cell:植物逆境硝酸根再分配调节作用 近期植物学研究权威期刊Plant Cell在线发表了植物生理生态研究所植物分子遗传国家重点实验室龚继明研究组最新研究成果:拟南芥NRT1.8基因介导的NO3-再分配在植物逆境胁迫耐受机理中起着重要的调节作用。 硝酸根(NO3-)是陆生植物最重要的氮源之一,它不仅是重要的营养物质, 近期植物...
It involves probing cells with light at wide angles, which unfortunately requires complex systems. Here we show an intriguing concept based on an inherent natural process for plants biology, i.e., dehydration, allowing us to easily obtain 3D-tomography of onion-epidermal cells' nuclei. In fact,...
Plant cells build nanofibrillar walls that are central to plant growth, morphogenesis and mechanics. Starting from simple sugars, three groups of polysaccharides, namely, cellulose, hemicelluloses and pectins, with very different physical properties are assembled by the cell to make a strong yet exten...
In plant cells, one connection stands out: the Hechtian strand which, during plasmolysis, reflects the existence of a physical link between the plasma membrane of the retracting protoplast and the cell wall. The Hechtian strand is part of a larger structure, which we call the Hechtian structure...
For localization of GFP-labeled BcCrh1 during infection, conidia were suspended in PDB medium and 10 μl droplets of spore suspension containing 1 × 104 conidia ml−1 were placed on onion epidermal cells and incubated at 20 °C in moist conditions. Samples were prepared for ...
transientexpression of monomeric red fluorescent protein-fused OsATL6 in onionepidermalcells confirmed the tonoplastlocalizationof OsATL6. When OsATL6 was expressed in Xenopus laevisoocytes,glutamineeffluxfrom the cell into theacidicbath solution increased. Under sufficient NH4+ supply, OsATL6 transiently...
Microprojectile bombardment into plant cells was first described by Sanford et al. [19] in 1987. At that time, tungsten particles of approximately 4 μm size were accelerated into onion epidermal cells and directly visualized using an inverted microscope. The original air pressure propulsion system...
The regions of the actin molecule that are involved in F-actin assembly are largely conserved, and it is likely that mammalian actin is able to incorporate into microfilaments in plant cells but there is no experimental evidence until now. Results Visualization of microfilaments in onion bulb ...
Subcellular localization analysis of MiTFL1 proteins. 35S::GFP-P1300 and 35S::GFP- MiTFL1s were localized in onion epidermal cells Full size image Phenotypic analysis ofMiTFL1overexpression inArabidopsis thaliana MiTFL1genes delay flowering inArabidopsis thaliana ...
To this end, 35S:CsPAO4-GFP vector was constructed and transformed into onion epidermal cells. After observation with fluorescence microscope, the control GFP was observed throughout the cell, whereas CsPAO4 was revealed to be localized to the periphery of plant cells, including cell wall, ...