As open-field conditions are highly complex, a greenhouse experiment was conducted to verify the open-field findings. Fungal discs were punched from the edge of a colony of fungus actively growing on PDA plates, and one-year-oldP. notoginsengwere inoculated by placing the fungal disc on the ...
The discovery of this phenomenon shows that the cross talk of two TM transport pathways in a plant cell is possible, at least for the essential TMs such as Cu. It is still unknown, however, whether such a transport pathway can function in plant cell toxic metal sequestration strategy and fo...
Afterward, the tissues were cleared in ethanol and stored in 20% (v/v) glycerol for photographing under a dissection microscope. Statistical analysis The number of independent experiments and replicates per experiment are indicated in the table headings and figure legends. Data were plotted as means...
ns not significant. Data were analyzed by two-way ANOVA multiple comparisons test with the Holm–Šídák method. ns,P > 0.05. Each design contains three independent experiments (n = 3). Data are presented
To understand processes regulating nutrient homeostasis at the single-cell level there is a need for new methods that allow multi-element profiling of biological samples ultimately only available as isolated tissues or cells, typically in nanogram-sized samples. Apart from tissue isolation, the main ...
plant. Microspores and pollen were isolated from buds of different sizes, stained with DAPI, and viewed under a fluorescence microscope using the UV light channel and normal light to determine the developmental stages of pollen. a1-a5 Flowers of various sizes. a1 10-12 mm. a2 18-22 mm. ...
Long and short term live cell imaging Videos were acquired on a compound light microscope using the audio video interleave (AVI) capture function in NIS-Elements AR software version 3.0. Long term live cell imaging (~72 h) experiments were carried out using window stage leaves where PCD was ...
[4] were able to characterize stem cell divisions in the root meristem for over a day on a standard microscope with customized tracking software. They tracked the root mostly in a single central z-plane at each time point, with the longest continuous experiment spanning 28 h. Another approach...
Crh proteins catalyze crosslinking of chitin and glucan polymers in fungal cell walls. Here, we show that the BcCrh1 protein from the phytopathogenic fungus Botrytis cinerea acts as a cytoplasmic effector and elicitor of plant defense. BcCrh1 is localize
Structural changes in the cell wall were followed by an increase in Young’s modulus (compressive stiffness of a solid material, expressed as the relationship between stress and axial strain) of the cell wall material. The data presented in this paper show in a novel way how storage under ...