primerDesignProgram.jsp was used to design primer pairs to introduce mutations into the AYVV CP in pEAQ-HT-D1-AYVVCP at residues 41 (R to A: KS125P and KS126P), at residue 48, (R to A: KS127P and KS128P), and at residue 59 (M to D: KS129P and KS130P); Supplementary ...
The ubiquitin E3 ligase complex containing Skp1, Cullin1, and the F-box protein EBF1 or EBF2 (SCFEBF1/2) target the degradation of EIN3, the master transcription factor in ethylene signaling. We coupled EBF1 and EBF2 to a number of cell type-specific promoters. Using phenotypic assays ...
The crystal structure of a major allergen from plants. Background: Profilins are small eukaryotic proteins involved in modulating the assembly of actin microfilaments in the cytoplasm. They are able to bind bot... KS Thorn,HE Christensen,RS Jr,... - 《Structure》 被引量: 386发表: 1997年 ...
Flagellin (and flg22) is the ligand for FLAGELLIN-SENSING 2 (FLS2), a receptor in plant cell membranes. FLS2 is similar in structure to, for example, the Pep receptors (PEPRs), which bind the endogenous plant elicitor peptides (Peps) [10]. Plants also employ endogenous signaling ...
In vitro cell culturing represents an alternative means for production of this drug, considering that natural populations of one of the most important sources, the yellow ginger (Dioscorea zingiberensis), are decreasing due to overexploitation, and agricultural production is problematic by reason of ...
One mechanism, activated in response to an elevated external Ca2+ level, entails calcineurin B-like (CBL) Ca2+ sensors and CBL-interacting protein kinases (CIPKs), which activate CAXs by phosphorylating a serine (S) cluster in the auto-inhibitory domain. The second pathway, triggered by ...
A ‘phenotype’ is the composite set of one or more observable characteristics associated with a given organism or cell, that results from the interaction of the genotype and the environment [7,8]. The separate characteristics that make up a phenotype are termed ‘phenes’ [9,10]. For ...
Relationship of the inhibition of cell migration with the structure of ginseng pectic polysaccharides. Carbohyd Polym. 2010;81(2):340–7. Article CAS Google Scholar Gao X, Zhi Y, Sun L, Peng X, Zhang T, Xue H, Tai G, Zhou Y. The Inhibitory Effects of a Rhamnogalacturonan Ι (RG...
In plants, the increased PC to PE ratio is a self-protective action that maintains the stability of the cell membrane under stress conditions [17]. The PC to PE ratio in L. chinensis was five times higher than that in Arabidopsis (Fig. 6D), suggesting that the membrane stability of L....
The cell surface receptor chitin elicitor receptor kinase 1 of Arabidopsis (AtCERK1) directly binds chitin through its lysine motif (LysM)-containing ectodomain (AtCERK1-ECD) to activate immune responses. The crystal structure that we solved of an AtCERK1-ECD complexed with a chitin pentamer ...