Using a combination of biochemical methods, live-cell imaging, high-resolution deconvolution and 3D-electron tomography (ET) analysis, we showed that the autophagosomal structures that accumulated in the Arabidopsis free1 mutants were largely unclosed autophagosomes in nature. Analysis of the FREE1 ...
Preparation of cell-free bacterial supernatant Col-0 (15–20 plants) plants were pregerminated on half-strength MS agar plate supplemented with 5 g l−1 sucrose. After two weeks, plants were submerged in 20 ml washed bacterial suspension (OD600 = 0.0005) in half-strength MS medi...
2d). While NDUP9 and QCR6 showed mostly hydrophobic interactions, those between QCR6 and NDUA11 were more electrostatic in nature. Lastly, the membrane site was a small interface of a couple of residues on NDUA11 and QCR8 (Fig. 2e). As in the O. aries and T. thermophila SC I +...
of cell markers for six model plants—Arabidopsis, rice, maize, soybean, tomato and tobacco—including experimentally validated marker genes, differentially expressed marker genes based on bulk RNA-seq data, and differentially expressed genes across specific cells identified by single-cell RNA sequencing...
acerina infection causes cell chloroplast membrane disruption in PNLs, crude exoenzymes of M. acerina whose expression was induced by exposure to PNL tissue (both in vivo and in vitro) or pectin (induced exoenzymes) were obtained, and their effects on plant leaves were investigated by the ...
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Empty CellStemNodePetioleLeafGr. pointOvaryTendrilNon-plant 8-class X X X X X X X X 4-class X X X X 3. Results In this section, the results of the experiments defined in section 2.4 are presented. First, in section 3.1, the results of the segmentation based on geometric data are...
Cell wall gels, produced directly in the NMR tube with perdeutero-dimethylsulfoxide, allowed the acquisition of well resolved/dispersed 2D13C–1H correlated solution-state NMR spectra of the entire array of wall polymers, without the need for component fractionation. That is, without actual ...
A 3D object may also be represented by a 3D array of cells, in which each cell (voxel) contains two possible values, indicating whether a voxel is occupied by the object or not. The most commonly used methods which result in such a representation are shape estimation methods [96] like Sh...
2d). More contiguous genome assemblies with higher contig N50 had more complete BUSCOs (P < 0.0088), and this was associated with the use of long reads in the assembly process (P < 0.0001; Fig. 2c–d and Extended Data Fig. 3). Despite the wide range of BUSCO completeness, ...