BMC Plant Biology BioMed Central Research article Open Access Cross-species EST alignments reveal novel and conserved alternative splicing events in legumes Bing-Bing Wang1,3, Mike O'Toole1, Volker Brendel2 and Nevin D Young*1 Address: 1Department of Plant Pathology, University of Minnesota, St....
3. VDAC sequences and structures 3.1. Sequences of the VDAC proteins Multiple sequence alignment (MSA) built using VDAC protein sequences from different species including animal, fungi and plant proteins revealed only a very low sequence identity ≤ 5% (Table 1), close to that found for a se...
2 and 3C–8), whereas cells carrying the pUGK4 vector were not immunolabeled (Figs. 1 and 3C). Thus, SARS-CoV-2 antigens were successfully expressed on the surface of L. plantarum SK156. Cellular immune response of murine macrophage induced by recombinant L. plantarum To initially ...
Then, cells were lysed using Dounce homogenizer method to release chromosomes and DNA. DNA was digested using DPNII, generating sticky ends. These ends were filled and labeled with biotin using DNA polymerase. The filled DNA ends were then ligated to form linked products. The biotin labeled Hi-...
After labeling has been achieved, these two labeled protein samples were digested with Lys-C, peptides were labeled in vitro with light (213CD2H) and heavy (2CD3) dimethyl groups. Then, the four different pseudo-isobaric labeled prepared samples were mixed in a 1:1:1:1 ratio and analyzed...
green color. Inter-molecular hydrogen bonding and π-π staking (aromatic) were labeled in magenta and yellow color respectively, and loops, helical structure, etc. were colored automatically, with images rendered for better presentation. Statistical analysis...
Pythium ultimum is a ubiquitous oomycete plant pathogen responsible for a variety of diseases on a broad range of crop and ornamental species. The P. ultimum genome (42.8 Mb) encodes 15,290 genes and has extensive sequence similarity and synteny with rel
Briefly, wild-type and mutated PYL1 and PYL10 proteins were labeled in assay buffer (50 mM HEPES, pH 7.5, 100 mM NaCl) using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). ABA in a range of concentrations (0.0001 μM to 4 mM) was incubated with 2 μM of labeled ...
[5,6,7]. ROS induce oxidative stress in plants, which affects growth characteristics and alters the redox status of plant cells [8,9,10]. Vegetables grown near industrial sites develop poorly due to the large amount of heavy metals in the soil [11]. Microorganisms play a key role in ...
Different metabolic compounds give pepper leaves and fruits their diverse colors. Anthocyanin accumulation is the main cause of the purple color of pepper leaves. The light environment is a critical factor affecting anthocyanin biosynthesis. It is essent