For wildtype and Tle3−/− conceptuses, Tle3+/− mice were crossed and pregnant females dissected at embryonic days (E) 8.5 through 17.5 (noon on the day of the vaginal plug was designated as E0.5). Notch2+/del animals have been previously described and have all but one ankyrin ...
for 5 min and centrifuged at 12,000×gfor 15 min. The supernatant was collected, and the protein quantity was quantified using Bradford Reagent (Sigma-Aldrich®, Milan, Italy) following the manufacturer’s instructions. Proteins were run in SDS-PAGE gel (10% of acrylamide for the analysis ...