Mouse monoclonal antibody against ubiquitin and GST were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal antibody recognizing PKM2-389 (a customer antibody) was obtained from Signalway Antibody (College Park, MD, USA). Mouse monoclonal antibodies for Flag, His and ...
The slides were then incubated with anti-KDM8 rabbit polyclonal antibody (homemade) at 1:100 dilutions overnight at 4 °C, followed by incubations with biotinylated secondary antibody and the ABC reagents in the Vectastain Elite kit and counter-stained with hematoxylin. The percentage of ...