The kit provides a means of performing kinase activity assays with recombinant murine PKCeta kinase. It includes active PKCeta kinase (supplied as a GST fusion protein), a biotinylated peptide substrate and a phospho-serine/threonine antibody for detection of the phosphorylated form of the substrate...
The non-radioactive PKC Kinase Activity Assay provides a safe, rapid and reliable method for screening of inhibitors or activators of PKC and for quantitating the activity of PKC in partially purified, purified or crude enzyme preparations.The PKC Kinase Activity Assay is based on a solid phase ...
Stage Two: PKC kinase activity assay 1. Add 2.5-5 µL of the BSA-kinase substrate (component C) to kinase sample prepared in stage one. 2. Add 5 µL of the ATP (component E) solution to initiate the kinase reaction at 37oC for 60 min with constant shaking or hand shake every ...
Protein content was measure in the lysates using the BCA method (Thermo Fisher Scientific, Rockford, IL, USA), and 1.5 μg of crude protein was used per assay. The amount of PKC kinase activity was measured in each sample using the PKC Kinase Activity Assay Kit (Abcam, Cambridge, U.K...
PKCε kinase activity in the cell lysate was determined by a PKC kinase activity kit according to the manufacturer’s protocol (ADI-EKS-420A, Enzo Life Sciences, Farmingdale, NY, USA), which is based on a solid-phase enzyme-linked immunosorbent assay that utilizes a specific synthetic peptide...
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产品编号: CY-1175 查看说明书 产品名称: CycLex PKC Super Family Kinase Assay Kit .0 订购此产品 供应商: MBL 规格: 96 wells 目录价: 7100 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址: 点击查看详细 打印此页 关闭此页 上一个:CycLex Aurora Family Kinase ...
人蛋白激酶C(PKC)elisa定量检测试剂盒英文名称:Human protein kinase C (PKC) ELISA KitELISA试剂为我司主营产品之一,产品皆严格按照相关标准进行 ,并经过了严格地反复地检测,我们以严谨的态度保证产品的质量,从而保证您的实验顺利进行。产品供货周期短,供货及时,且我司还提供完整的售前和售后服务。别名:人蛋白激酶...
PKC-delta has a biological activity which is closely related to Pkc1, and PKC-delta activates the Pkc1-mediated pathway through an activation of the Bck1 kinase. PKC-delta appears to play a critical role in growth control of yeast and mammalian cells. Suppression experiments suggest that PKC-...
Kinase inhibition assays: The inhibition of PKCβII, PKCα, PKCε, or PKCγ activity by enzastaurin is determined using a filter plate assay format measuring 33P incorporation into myelin basic protein substrate. Reactions are done in 100 μL reaction volumes in 96-well polystyrene plates with ...