Rabbit polyclonal antibody against PKC alpha protein. Immunogen region is C-terminal. Specificity is as follows for the reactive species: H:Y657, M:Y657, R:Y657.应用类型 ELISA, WB免疫原 PKC alpha来源宿主 兔反应性 人,小鼠,大鼠保存建议 建议收到该PKC alpha (Phospho-Tyr657) Antibody产品后,...
PKC alpha Antibody (MA1-157) in IF Immunofluorescent analysis of PKC alpha (green) in SH-SY5Y and MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minute...
Based on 100% sequence identity, this antibody is predicted to react with Rat and Bovine 靶标信息 The PKC family of serine/threonine kinases, including PRKCA (PKC alpha), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been ...
Specificity Phospho-PKC α (T638) Polyclonal Antibody detects endogenous levels of PKC α protein only when phosphorylated at T638. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol,...
Specificity Phospho-PKC α (Y657) Polyclonal Antibody detects endogenous levels of PKC α protein only when phosphorylated at Y657. Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen Form Liquid in PBS containing 50% glycerol,...
Western blot analysis of MA1-157 detects an ~80 kDa protein in human, bovine, non-human primate, mouse, rat, dog and rabbit cells. In Jurkat, rat brain and MDCK cell lysates, a weak unknown band at ~140 kDa is also detected. MA1-157 shows specificity to PKC alpha and is non-reactiv...
Western blot analysis of extracts from Jurkat cells, using PKD1/PKC mu (Ab-910) Antibody. The lane on the right is treated with the synthesized peptide. Related products SHIP1 (Ab-1020) Antibody SGK (Ab-78) Antibody PP1-alpha (Ab-320) Antibody ...
and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein...
( alpha, beta 1, beta 2, and gamma ) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms (δ, epsilon, eta, and theta ), which are calcium-independent but are still regulated by PS, ...
Sandwich ELISA, Double Antibody Detection Wavelength OD450 Reaction Duration 4 hours Range 0.313-20ng/ml Sensitivity 0.188ng/ml Storage 2-8°C(Sealed), Don't cryopreserve. Specificity Specifically binds with PKCα , no obvious cross reaction with other analogues. ...