PKC alpha Antibody (MA1-157) in IF Immunofluorescent analysis of PKC alpha (green) in SH-SY5Y and MCF7 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minute...
and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of pro...
Immunogen The antiserum was produced against synthesized peptide derived from human PKC alpha. Immunogen Region C-terminal Clonality Polyclonal Application dilutions WB: 1:500~1:1000 ELISA: 1:5000 Specificity PKC alpha (Ab-657) Antibody detects endogenous levels of total PKC alpha protein. Purificatio...
and substrate specificity. PKC alpha, beta I, beta II and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This ...
产品英文名称:phospho-pkcalpha/beta ii (thr638/641) antibody #9375 货号:pro_23839产地:cellsignal 规格/价格:询价 产品详细说明: product s - ca / camp / lipid signaling datasheet pdf msds pdf protocols applicationsreactivitysensitivitymw (kda)source ...
specificity a synthetic phospho-peptide corresponding to residues surrounding thr638 of human pkc was used as immunogen. the antibody only detects pkc alpha phosphorylated on threonine 638. predicted to cross-react with rat, bovine and chicken, based on sequence homology. ...
Specificity of single LIM motifs in targeting and LIM/LIM interactions in situ. Genes Dev. 10, 289–300 (1996). Article CAS PubMed Google Scholar Flick, M. & Konieczny, S. The muscle regulatory and structural protein MLP is a cytoskeletal binding partner of bI-spectrin. J. Cell Sci....
( alpha, beta Ι, beta ΙΙ, and gamma ) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms (δ, epsilon, eta, and theta ), which are calcium-independent but are still regulated by PS, DAG, or phorbol esters; and ...
Antibody SpecificitySpecies SpecificityThe suprachiasmatic nucleus (SCN) is the circadian pacemaker in mammals and contains a network of arginine-vasopressin-immunoreactive (AVP-ir) neurons. AVP-recipient cells contain the V1a class of receptors linked to phosphoinositol turnover and protein kinase C (...
C Flow cytometry analysis of cells isolated from neurospheres treated under the different conditions indicated and labelled with anti-Ki67 antibody; left panel: forward/side scatter; central panel: cell count vs. fluorescence intensity in the four different condition; right panel graph shows the ...