Fig. 1 The cAMP signaling pathways. The extracellular cAMP can be converted into AMP by plasma membrane-anchored ecto-PDE, followed by the conversion of AMP into adenosine by CD73. Ligands-engaged Gs- or Gi-coupled receptors may stimulate or inhibit cAMP synthesis, respectively. The ABCC transp...
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Vollum Institute, Oregon Health and Science University, Portland, OR, USA Lei Ma, Julian Day-Cooney, Michael A. Muniak, Maozhen Qin, Tianyi Mao & Haining Zhong Department of Neurosurgery and Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Palo Alto, CA,...
Rabbit anti-Synaptophysin and mouse anti-NeuN antibodies were obtained from Abcam (Cambridge Science Park, Cambridge, UK). A rabbit anti-Synapsin I antibody was purchased from Proteintech (Rosemont, IL, USA); an FD Rapid GolgiStain Kit was obtained from FD Neurotechnologies, Inc. (Guilford, MD,...
For quantitative histology, we propose a principled and veridical data science methodology that re-orients the perspective from image-level analysis to neuron-level representations of cortical areas. The individual neurons themselves are the unit of study, rather than the constituent pixels of the ...
Extensive research efforts spanning three decades have been devoted to investigating the fundamental science of vanadium oxide and exploring its potential in fields such as ion batteries, water splitting, smart windows, supercapacitors, sensors, and beyond. The current state-of-the-art in the ...
HCT116 cell line is purchased from Procell Life Science Technology (Wuhan, China). The cells were cultured in DMEM (Gibco) containing 10% fetal bovine serum (FBS, Gibco). For cell passages, the medium should be aspirated and washed once with PBS to remove serum and dead cells. Then 1 ...
(the primer sequences used are shown in Additional file 1: Table S1), and then the cells were selected with growth medium containing puromycin (3 μg/ml; CAS. 58-58-2; Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) for 48 h to obtain stable and highly pure clones...
Now you tell me, what is pH? Hopefully, you’re clued in with a cogent answer now and can confidently apply what you’ve learned in this article to your day-to-day research! Still confused? Don’t hesitate to post your questions in the comments section. ...
Oct 31, 2024 Reformation Day Symphony Oct 2, 2024 In God Calls Samuel, I preach on communion and communication. We long for communion with God; we find it when we attune to his word in Christ—listening and responding. Matt 4v4:“‘Man shall not live by bread alone, but by every ...