The subject is the acidity of organic compounds, which are employed as pH indicators, particularly in acid-base titrations. The indicators used are methyl orange, bromophenol blue and bromocresol green in aqueous medium. The influence of colloidal systems on the equilibrium is evaluated by the pKa...
Interestingly, we found that only displacement of PKA-II reversed the effects on autophagy, providing a link between cAMP/PKA microdomains and this process. Materials and methods Reagents Forskolin (FSK), H-89 dihydrocloride (H89), Calyculin A (CalA), 3-isobutyl-1-methylxantine (IBMX), ...
cAMP-elevating agents, such as forskolin, can not only induce cancer cell death, but also directly inhibit cell proliferation in some cancer types. In this review, we summarize current understanding of cAMP signaling in cancer biology and immunology and discuss the basis for its context...
I'm a student and I'm taking a "general chemistry" course. in the last lab meeting i had - i used spectroscope to find the Absorbency of orange methyl indicator in different wave length. (500; 510; 520;530) and in differents materials (acid; base; pH=3; pH=4) and i got 4 resu...
Results: PKA phosphorylation of Shp2 at Thr-73/Ser-189 inhibits tyrosine-phosphorylated ligand binding and PTP activity. Conclusion: AKAP-Lbc integrates PKA and Shp2 signaling, and AKAP-Lbc-associated Shp2 activity is reduced in hypertrophic hearts following chronic β-adrenergic stimulation. Significa...
1b). Notably, addition of 10 μM cilostazol or 300 μM cAMP largely abrogated the BafA1-induced changes in lysosomal pH (Fig. 1b). To further confirm this effect, we turned to other pH-sensitive fluorescent dyes, acridine orange (AO) and Lysosensor Yellow/Blue DND-160 (DND-160). The...
Figure 3. Effect of pH on the 19F signal of 4F3HB in the presence of WT 3HB6H at 4 °C. A, 19F signal of 4F3HB bound to 3HB6H at pH 6.5 (orange spectrum) and pH 9.0 (light blue spectrum), and the simulations of the NMR lines are shown in red and blue lines for the pH...
the role of cAMP/PKA microdomains has not been tested. Here we show not only that the effects on autophagy of the same cAMP elevation differ in different cell types, but that they depend on a highly complex sub-compartmentalization of the signaling cascade. We show in addition that, in HT...
Using crystal structures, we show that the phosphorylation hotspot domain of RyR2 embraces the PKA catalytic subunit, with an extensive interface not seen in PKA complexes with peptides. We trapped an intermediary open-form PKA bound to the RyR2 domain and an ATP analog, showing that PKA can ...
In addition, we evaluated and compared the CS level of each cancer cell type with that of derived clones (Fig. 3C and D). Indeed, no significant difference was observed between control cells and IF1-depleted clones. Cytofluorometric analysis performed by using the nonyl acridine orange (NAO)...