The subject is the acidity of organic compounds, which are employed as pH indicators, particularly in acid-base titrations. The indicators used are methyl orange, bromophenol blue and bromocresol green in aqueous medium. The influence of colloidal systems on the equilibrium is evaluated by the pKa...
cAMP-elevating agents, such as forskolin, can not only induce cancer cell death, but also directly inhibit cell proliferation in some cancer types. In this review, we summarize current understanding of cAMP signaling in cancer biology and immunology and discuss the basis for its context...
I'm a student and I'm taking a "general chemistry" course. in the last lab meeting i had - i used spectroscope to find the Absorbency of orange methyl indicator in different wave length. (500; 510; 520;530) and in differents materials (acid; base; pH=3; pH=4) and i got 4 resu...
Interestingly, we found that only displacement of PKA-II reversed the effects on autophagy, providing a link between cAMP/PKA microdomains and this process. Materials and methods Reagents Forskolin (FSK), H-89 dihydrocloride (H89), Calyculin A (CalA), 3-isobutyl-1-methylxantine (IBMX), ...
1b). Notably, addition of 10 μM cilostazol or 300 μM cAMP largely abrogated the BafA1-induced changes in lysosomal pH (Fig. 1b). To further confirm this effect, we turned to other pH-sensitive fluorescent dyes, acridine orange (AO) and Lysosensor Yellow/Blue DND-160 (DND-160). The...
Figure 3. Effect of pH on the 19F signal of 4F3HB in the presence of WT 3HB6H at 4 °C. A, 19F signal of 4F3HB bound to 3HB6H at pH 6.5 (orange spectrum) and pH 9.0 (light blue spectrum), and the simulations of the NMR lines are shown in red and blue lines for the pH...
Answer to: Phenolphthalein has a pKa of 9.7 and is colorless in its acid form and pink in its basic form. For pH= 4.7 calculate [In-]/[HIn]. By...
Results: PKA phosphorylation of Shp2 at Thr-73/Ser-189 inhibits tyrosine-phosphorylated ligand binding and PTP activity. Conclusion: AKAP-Lbc integrates PKA and Shp2 signaling, and AKAP-Lbc-associated Shp2 activity is reduced in hypertrophic hearts following chronic β-adrenergic stimulation. Significa...
The indicators used are methyl orange, bromophenol blue and bromocresol green in aqueous medium. The influence of colloidal systems on the equilibrium is evaluated by the pKa. The colloids employed are surfactants like sodium dodecyl sulfate, cetyl-trimethylammonium bromide and a polymeric non-ionic ...
The obtained extracts were applied for acid-base titration, and the pKavalue was determined and compared to bromothymol blue and methyl orange, conventional acid-base indicators. Furthermore, the pKaof some naphthoquinones that occur inEuclea natalensisroot were predicted using MolG pKa web server ...