Following the transfection of specifically synthesized small interfering RNA (siRNA) into colon cell lines, we used Hoechst staining assay to measure the ratio of cell death in the absence of circPIP5K1A. Moreover, we also used the Transwell assay to assess the migratory function of colon cells...
Furthermore, the failure to restore Wnt signalling activity by the E129A mutant in cells where PIP5K1 was silenced by siRNA (Fig. 4b) further supports the importance of DVL–PIP5K1 interaction in the Wnt signalling. PIP5K1A and PIP4K2B share the same catalytic residues and signature motifs...