a Schematic showing the tools (elec- trophysiology and MD simulation) used to investigate the micro- and macroscopic properties of TRPC3 interaction with PIP2. The closed state cryo-EM structure of TRPC3 is shown in the centre (PDB: 6CUD8). b Whole-cell net current density recorded from ...
To determine the molecular basis of Frizzled 7 and PIP2binding by the PDZ domains of syntenin, we purified various constructs of syntenin (amino acids 107–275; 108–275; 113–273; 113–270; 107–192; 108–192; and 196–275) and tried to co-crystalize various combinations of bipartite an...
This co-operative binding of the PDZ2 in isolation was univocally demonstrated by the combination of our X-ray structure, the SPR data and the mutational analysis. Moreover, the data support a model where the PDZ2 is on its own responsible for the co-operativity of the tandem. Indeed, ...
PaIdPv5aKnpcirnogteionuframunildieesrsftraonmdiangcoomfmthoen evolutionary divergence of different members of the PIP4K/PIP5K protein families from a common ancestor and also to identify novel molecular features in these proteins that are distinctive characteristics of a particular family or subfamily ...