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Current activation was sigmoidal, and full activation was reached from 0 mV step with half-maximal current activation potential (V1/2) of −30.5 ± 0.5 mV (Fig. 4a–d, Table 2). Due to this increase in outward K+ currents, these cells also displayed hyperpolarized Vm compared to cells...
Here, we performed a series of Targeted molecular dynamics simulations on the full-length Kir2.1 channel and, for the first time, were able to achieve the transition from the closed to the open state. Our data show that with the upward motion of the cytoplasmic domain (CTD) the structure ...
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Current activation was sigmoidal, and full activation was reached from 0 mV step with half-maximal current activation potential (V1/2) of −30.5 ± 0.5 mV (Fig. 4a–d, Table 2). Due to this increase in outward K+ currents, these cells also displayed hyperpolarized Vm compared to cells...
I just smiled and signed his 60-day action plan, walked out and called HR to verify the steps that would be needed to have it removed from my personnel file later. Sure enough, the FSM was gone to Corporate in a year, and I submitted the proper form to have the black spot removed....
The PIPL’s definition is more descriptive but does not include a full list as the GDPR does. Article 28 of the PIPL specifies that sensitive PI is “PI that is likely to result in damage to the personal dignity of any natural person or damage to his or her personal or property once...
The full-length coding sequence of AtPIP1;4 was isolated from Col-0 and retested by Y2H in crosswise combinations with Hpa1 or Hpa1∆NT as mutual bait and preys. This crosswise assay indicated AtPIP1;4 interaction with both Hpa1 and Hpa1∆NT (Supplementary Fig. 2). Proteins were ...
4.2. RNA Interference For the RNAi of ppk-1, we used the full coding sequence of 1836 bp (F55A12.3) from C. elegans cDNA. The primers used were as follows: forward 5 -CCCGGGATGGCTTCTCGGTCCAC-3 and reverse 5 -CCATGGTCAAGCGACAGGTGTGT-3 . The sequence was cloned to a L4440 feeding...