1.2 mM EDTA, 167 mM NaCl, 0.01% SDS, 1.1% Triton-X100) (667 µL for ChIP intp63−/−mutants). Then, chromatin was sonicated in a Covaris M220 sonicator (duty cycle 10%, PIP 75 W, 100 cycles/burst, 10 min) or in a Bioruptor 200 sonicator (High intensity...
Bargellini PL: An Engineer's Review of Antonio Meucci's Pioneer Work in the Invention of the Telephone. Lecture at Philadelphia IEEE Section meeting, Sept. 15, 1992. Also printed in Technology in Society, Vol. 15, n. 4, p. 409-421, 1993....
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The cassettes plus open reading frames were inserted between the NdeI/EcoRI sites of pLJM1–EGFP, a puromycin-selectable lentiviral vector (Addgene, plasmid no. 19319)65. The inserts replaced the CMV–EGFP construct in the vector and the resultant plasmids were notated with the suffix pLJM1. ...
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Mine tailings are man-made environments characterized by low levels of organic carbon and assimilable nitrogen, as well as moderate concentrations of heavy
E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages 67-88 and Armstrong, “The First Decade of Maize Transformation: A Review and Future Perspective” (Maydica 44:101-109, 1999). In addition, expression vectors and in vitro culture methods for plant cell or tissue transformation and ...
(2006) Determinants of plant reproductive susceptibility to habitat fragmentation: review and synthesis through a meta- analysis. Ecol. Lett. 9, 968–80. Aguilar R., Quesada M., Ashworth L., Herrerias-Diego Y. & Lobo J. (2008) Genetic consequences of habitat fragmenta- tion in plant ...
E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages 67-88 and Armstrong, “The First Decade of Maize Transformation: A Review and Future Perspective” (Maydica 44:101-109, 1999). In addition, expression vectors and in vitro culture methods for plant cell or tissue transformation and ...