Sections were systematically sampled using the stereology software newCast (Visiopharm) and cell proliferation was quantified as the number of Ki67+ labeled beta-cell/acinus-cell nuclei profiles divided by the total number of beta-cell/acinus-cell nuclei profiles counted and expressed as the number ...
Livers in complemented chimeric fetuses in both species were comprised of eGFP-labeled donor-derived cells and survived beyond the previously observed time of HHEX KO embryonic lethality. Conclusions This work demonstrates that loss of liver development in the HHEX KO can be rescued via blastocyst ...
Southern hybridization was performed on the S1-PFGE gel using DIG High Prime DNA Labeling and Detection Starter Kit II (Roche, Germany) with digoxin- labeled DNA probes specific forvanA, followed by manufacturer’s protocol. Plasmid sequencing The whole genome of Efm4 includingvanA-harboring plasmid...
A subsample of feed was collected from each feed bag (~ 50 g) and pooled for each diet during the diet preparations. The feed samples were stored at -20 °C until further analysis. Fresh fecal samples were collected in pre-labeled 50 mL falcon tubes (VWR Radnor, PA) from the rectum...
(p) level. The taxa names were labeled by the colour code (magenta) to indicate the abundance of the taxa significantly differed between diet treatments (P < 0.05). The normalized relative abundance of bacterial taxa from illumina-sequenced 16 S rRNA sequences in each sample is ...
“integrative”claimsofalternativepracticesseriously:pletedatleastfouryearsofveterinaryschool.Thesamegoesforsupplements;justbecausesomething’slabeled“natural”doesn’tmeanthatit’snotnaturallytoxictoyourdog,orthatit’snotcontraindicatedwithanothersupplementormedicationshe’staking.29.HOWWILLNEUTERINGORSPAYINGAFFECTMY...
00 on the observation days was measured in seconds. A piglet was labeled as feeding in a video frame when their nose or head was observed to be partially or completely in, above, or touching the creep feeder. Piglets that met these criteria but were clearly lying down were not counted as...
Furthermore, the Bonferroni correction can lead to an overcorrection and can be too conservative, this can result in a limited number of labeled associationp-values that meet the standard across the genome. This can lead to a high false-negative rate. To address this issue, the false-discover...
This study evaluates the capacity of a bread enriched with fermentable dietary fibres to modulate the metabolism and nutrients handling between tissues, gut and peripheral, in a context of overfeeding. Net fluxes of glucose, lactate, urea, short chain fa
Fecal samples were collected from six-month-old herd pigs. Clean cotton swabs were used to gently stimulate defecation from the anus of the pigs, and fresh pig feces were scooped into 2 mL sterile centrifuge tubes. The samples were labeled, immediately frozen in liquid nitrogen, and stored. ...