(Figure 27-8). After sharp incision of the skin, the subcutaneous tissue is dissected bluntly. Thetunica vaginalisis also isolated by blunt dissection (seeFigure 27-8). The tunica vaginalis should be kept intact, because this will keep the intestine contained. While external pressure is put ...
Dissection techniques were used to prevent mechanical interactions between the LM and CM; also, the colonic segment was passed through a partition to prevent mechanical disturbances created by a peristaltic wave in the bulk of the colon from influencing the end from which recordings were made.3...
The dissection accuracy of the tissue samples was confirmed by histological analysis of adjacent tissue. Samples were sequenced with an average depth of 165.5 million reads (Additional file 2), and read counts were normalized (protein-coding transcript per million (pTPM) for visualization, and norma...
Moreover, this DIC pig line can also be used as a basic tool to develop a single transgene integration strategy that allows the generation of spatiotemporal gene knockout lines, empowering the preclinical replication of complicated diseases and more refined gene function dissection. DIC pigs with ...
Thanks to Toni Reverter for insightful discussions, to all people who helped in the dissection (Irina Garcia-Ispierto, Cristina Andreu, Llibertat Tussell, Sònia Tort, Sebas Morán and Carlos López-Plana), to J. Estellé for sharing unpublished QRT-PCR primers, to the personnel in the microa...
For this reason, we chose to undertake a longitudinal dissection of a single commercial pig unit, which included three group in-feed antibiotic treatment regimens and a partial depopulation event. Measured AMR genes consistently high across the unit Although previous work has demonstrated high baseline...
In order to limit the duration of warm ischemia, dissection of the graft was performed on the donor animal with respect for the following anatomic features: -absence of intestinal attachment to the posterior parietal peritoneum, -anti-clockwise torsion of 360掳 of the intestine around the superior...
In the two cadavers where neuronal tissue was not found, poor dissection, larger presence of perivascular fat, and connective tissue in the collected sample might have played a role too. Being a descriptive cadaveric study, we did not look at the efficacy of the technique. In humans, the ...