Piezo1在不同类型的细胞中诱导 MA电流，克隆N2A细胞中Piezo1基因到pIRES2-EGFP载体中，在转染12-48小时后，在全细胞模型中记录了绿色荧光蛋白(GFP)阳性细胞的 MA电流。在N2A、HEK293T和 C2C12细胞系中，Piezo1过表达，MA电流-电压关系与内源性N2A细系中MA电流相似。用离子选择性的方法研究了Piezo1过表达的三...
Several members of the type IV subfamily of P-type adenosine triphosphatases (P4-ATPases) that are complexed with an auxiliary CDC50 subunit act as a phospholipid flippase that translocates the cell surface-exposed PS to the inner leaflet of the plasma membrane3,4,8,16,17,18. In mammals, ...
dPiezo chimeras were cloned into pIres2-EGFP vector and all mPiezo1 chimeras and mutants were cloned into pcDNA3.1( À ) vector, which was modified to include an IRES-EGFP element. mPiezo2 (E2416K) was cloned in pCMV-Sport6 vector. Cell culture and transient transfection. Human embryonic...
Piezo1和Piezo2诱导MA电流 Piezo1在不同类型的细胞中诱导 MA电流,克隆N2A细胞中Piezo1基因到pIRES2-EGFP载体中,在转染12-48小时后,在全细胞模型中记录了绿色荧光蛋白(GFP)阳性细胞的 MA电流。 在N2A、HEK293T和 C2C12细胞系中,Piezo1过表达,MA电流-电压关系与内源性N2A细系中MA电流相似。用离子选择性的方法...
OPEN SUBJECT AREAS: EXPERIMENTAL MODELS OF DISEASE ION TRANSPORT BREAST CANCER MECHANISMS OF DISEASE Received 10 September 2014 Accepted 23 December 2014 Published 10 February 2015 Correspondence and requests for materials should be addressed to W.S. (W. Schreibmayer@gmx. at) * Current address: ...
Cells transfected with the Piezo1-myc-2422-pIRES-EGFP construct were washed once with pre-warmed culture medium and then incubated in warm medium with 1:50 anti-myc antibody (c-myc 9E11, Santa Cruz Biotech) for 20 min at 37 °C. Cells were then washed 2 Â with warm media and 1 ...