15939-1-AP targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples. 经测试应用 WB, IHC, IF/ICC, ELISA Application Description 文献引用应用 WB, IHC, IF, CoIP 经测试反应性 human 文献引用反应性 human, mouse, rat, pig, ...
The following primary antibodies were used in this experiment: Piezo1 (Proteintech Group, Inc.; cat. no. 15939‐1‐AP; 1:2,000), MMP3 (BOSTER Biological Technology, Ltd.; cat. no. BM4074; 1:500), MMP13 (Proteintech Group, Inc.; cat. no. 18165-1-AP; 1:1,000), SOX9 (...
Pri- mary antibodies used for incubation were as follows: Piezo1 (1:200, 15939-1-AP, Proteintech), H3K9me3 (1:500, 13969, Cell Signaling Technology), AGR2 (1:200, AF6068, R&D Systems), and Mucin2 (1:200, GTX100664, GeneTex). DAPI (D9542, Sigma, Millipore, St. Louis) was ...
The primary antibodies used were: PIEZO1 (1:200 dilution; catalog number: 15939-1-AP), nuclear factor-kappa B (NF-κB) p65 (1:200; 10745-1-AP), endomucin (1:200; 67854-1-Ig), and cluster of differentiation (CD)31 (1:3000 28083-1-AP). The next day, sections were washed four...
(Pierce). Anti-Piezo1-antibody was from Proteintech (catalog number 15939-1-AP, 1:200), anti-GAPDH, anti-FGFR1 and anti-AKT antibodies were from Cell Signaling Technology (#2188,#9740 and #9272, respectively, 1:500) and anti-FGF1 antibody was from Santa Cruz Biotechnology (sc-55520, 1...
Anti-Piezo1 (15939-1-AP), anti-COX2 (12375-1-AP), anti-GPX4 (67763-1-Ig), anti-ACSL4 (22401-1-AP), anti-ZO-1 (21773-1-AP), anti-Occludin (27260-1-AP), anti-Claudin-1 (13050-1-AP), anti-AMPK (10929-2-AP), anti-mTOR (20657-1-AP), anti-p-mTOR (67778-1-Ig), an...
The PIEZO1 (RRID: 15939-1-AP), Bcl2 (RRID: 26593-1-AP), and IBA1 (RRID: 66,827-1-lg) antibodies were purchased from Proteintech Bio Co., Ltd. (Wuhan, China). Aquaporin 4 antibody [EPR7040] (RRID: ab128906) was purchased from Abcam Trading Co., Ltd. (Cambridge, UK). NeuN ...
Piezo1 via Piezo1 inhibitor, GsMTx4. TUNEL staining and flow cytometry determined the apoptosis rate of periodontal tissue and PDLFs in rats. Expression of Piezo1, p-p38 and ERK1/2 was analysed by immunofluorescence assay and western blotting. Piezo1 inhibitor GsMTx4 relieved the increased ...
Sections (5 μm) were incubated with Piezo1 antibody (1:100; 15939-1-AP; Proteintech, China) and AE1/AE3 antibody (1:100; ab86734; Abcam, UK) overnight at 4 °C followed by 594-conjugated Goat Anti-Mouse IgG (H + L, 1:100; ABclonal, China) and FITC Goat Anti-Rabbit IgG (...
Proteins were transferred on a polyvinylidene difluoride membrane, and PIEZO1 protein was detected with a specific antibody (15939-1-AP, Proteintech). GAPDH was used as a loading control and detected with a specific antibody (ab8245-100, Abcam). See Supplementary Fig. 6 showing the uncropped ...