本品是一种常用的phosphatidylinositol 3-kinase (PI3K)抑制剂。LY294002可以通透细胞,特异性抑制PI3K,抑制PI3K/Akt信号途径,包括常见的抑制Akt磷酸化等。LY294002对于纯化的PI3K的IC50为1.4 μM。 CAS:154447-36-6 分子式:C19H17NO3 分子量:307.3 纯度:>98% ...
用100nM Akt抑制剂MK-2206抑制Akt激活可以显著消除AT1R和ETAR-IgG对 HMEC-1s损伤修复的阻碍作用(图3),这表明AT1R和ETAR-IgG通过激活Akt阻碍HMEC-1s损伤修复。 图3. AT1R和ETAR-IgG通过激活Akt阻碍HMEC-1s损伤修复 Akt通常促进细胞增殖和迁移[15...
(AKT)/mechanistic target of rapamycin (mTOR) signaling pathway is prevalently over-activated in human cancers and contributes to breast cancer (BC) growth, survival, proliferation, and angiogenesis, which could be an interesting therapeutic target. This review summarizes the PI3K/AKT/mTOR signaling ...
Stained cell pellets were washed again before staining with a secondary antibody (anti-rabbit Alexa 647). Events were subsequently acquired on a Canto II flow cytometer (BD Bioscience), and analysed using FACS Diva. MFI of pAKT in B cells was plotted against the anti-IgD concentration, which...
Finally, we found that low dose of anlotinib downregulated PD‐L1 expression only in VECs via inhibiting the PI3K/AKT pathway; however, the same effect was not observed in cancer cells. To sum up, our findings revealed that upregulation of PD‐L1 via activation of the PI3K/AKT ...
天津市 器官移植临床医学研究中心,天津 300192) 调节性 T 细胞(regulatory T cell,Treg)与 Th17 细胞间的相对平衡是实现器官移植术后免疫耐受的 关键因素.PI3K/Akt/mTOR 通路是调节 Treg/Th17 平衡的作用机制之一,对二者的分化,功能和增 殖均有重要影响.本文就 PI3K/Akt/mTOR 通路在 Treg/Th17 中的作用进行...
室温下用山羊血清工作液(中山金桥)封闭30 min后,用鼠单克隆抗PI3K、兔多克隆抗体p-PI3K(Thermo Fisher Scientific)、兔单克隆抗体AKT(Cell Signaling Technology)、兔单克隆抗体p-AKT(Cell Signaling Technology)等分别孵育,4℃过夜。第1...
(e) EGF-dependent MKRN1 ubiquitination is reduced by AKT ablation. ME-180 cells transduced with siAKT1 were treated with EGF (100 ng ml−1), followed by MG132 (10 μM) for 4 h. The lysates were immunoprecipitated using an anti-MKRN1 antibody, followed by immunoblotting with...
Only one of the PI3K-AKT-regulated KSRP targets, hnRNPA/B, is very weakly immunoprecipitated by anti-AUF1 antibody (Figure 1C and Additional file 5). Conversely, anti-hnRNPA1 antibody efficiently immunoprecipitates KSRP target transcripts (Figure 1C). Both AUF1p45 and hnRNPA1 bind very weakly...
The phosphatidylinositol 3-kinase (PI3K)/Akt pathway plays a crucial role in various cellular processes and is aberrantly activated in cancers, contributing to the occurrence and progression of tumors. Examining the upstream and downstream nodes of this