中文: 欧文氏菌AEBL002菌株八氢番茄红素脱氢酶基因保守序列的克隆与同源性分析更详细... 英文: Induction of Phytoene Desaturase (PDS) Gene Silencing in the Agrobacterium-mediated Transient Expression System 中文: 运用农杆菌介导的瞬时表达体系研究PDS基因沉默(英文)更详细......
One important target site for bleaching herbicides is the enzyme phytoene desaturase catalysing the desaturation of phytoene in zeta-carotene. This enzymatic reaction can be inhibited by norflurazon or fluridone. We have transformed tobacco with a mutated cyanobacterial phytoene desaturase gene (pds) ...
甜瓜果实八氢番茄红素脱氢酶基因(CmPDS)的克隆与表达%Cloning and Expression of Phytoene Desaturase Gene (CmPDS) from Fruit of Muskmelon 甜瓜PDS基因克隆表达以甜瓜自交系'M01-3'果实cDNA为模板,采用RT-PCR和RACE技术,克隆了甜瓜果实八氢番茄红素脱氢酶(PDS)基因cDNA全长序列,命名为CmPDS(基因注册号:KC50...
Phytoene desaturase (PDS) is an upstream key enzyme of carotenoid metabolic pathway inDunaliella bardawil. This research attempts to clone thePdsgene and its promoter and terminator fromD. bardawiland identify the promoter activity. Using LA-PCR based genomic walking approach and semi-nested PCR ...
gene stacking efforts through successive transformation or conjugation. We performed UV-mutagenesis ofP.tricornutumto obtain mutations in the phytoene desaturase (PDS) gene, conferring resistance to the bleaching herbicide norflurazon. Two mutants displaying high tolerance to norflurazon and carrying unique ...
among other species. The strategy to mutate and knockout the phytoene desaturase gene (PDS) by CRISPR/Cas9 has been widely applied to quickly demonstrate the feasibility of CRISPR/Cas9 since its mutation causes photobleaching or albino phenotype. Through transient expression assays or transformation met...
Fig. 1.Simplified carotenoid biosynthetic pathway in plants. Phytoene desaturase (PDS) and ζ carotene desaturase (ZDS) each catalyse the sequential introduction of two double bonds (positions circled). The subsequent cleavage of the product to vitamin A occurs only in animals and fungi. Abbreviation...
It has been proposed for decades that chaperones control phytoene desaturase (PDS) activity. The soluble, inactive forms of PDS proteins were demonstrated to be part of a large complex associated with Hsp70 or the chloroplast 60-kDa chaperonin (Cpn60) in the stroma (Al-Babili, von Lintig, ...
We identified a number of ripening-related proteins, of which several are involved in carotenoid biosynthesis, including carotenoid isomerase (CRTISO), PSY1, and phytoene desaturase (PDS), and the levels of these proteins were higher in the ppsr1 mutant fruit (Supplementary Data 1), consistent ...
Desaturation reactions catalysed by PDS and ζ-carotene desaturase (ZDS) in higher plants are also important in controlling flux through the pathway and these require coupled electron transfer with a plastidial terminal oxidase (PTOX) and plastoquinone, as has been proposed for D. salina [21]. ...