图3.Extreme fidelity for demanding PCR Relative fidelity values of different DNA polymerases. Phusion DNA Polymerases have extremely low error rates. The error rate, determined by a modified lacI-based method, is approximately 50-fold lower than that of Taq DNA polymerase and 6-fold lower than ...
Fidelity = 1/error rate. 图4.Less enzyme - superior yieldA 3.8 kb fragment from human beta globin gene was amplified with three different DNA polymerases. Phusion DNA Polymerase was able to amplify the 3.8 kb genomic fragment with a combined annealing and extension step of only 1 minute, ...
LabAid: Phusion High-Fidelity DNA Polymerases Technical Note: Measuring error rates of high-fidelity DNA polymerases by NGS Product Information User Guide: Phusion High-Fidelity DNA Polymerase User Guide: dNTP Mix, 10mM each, molecular biology grade订购...
Phusion100 High Fidelity DNA Polymerase Master Mix(2X)聚合酶master mix 为高效 PCR 扩增酶的直接使用混合物,Phusion100 DNA 聚合酶的错配率比Taq低100倍、比Pfu低12倍,是克隆和其他需要高保真度的应用的推荐选择。与其他 DNA 聚合酶相比,Phusion100 DNA 聚合酶性能稳定,实验方案耗时少(即使存在 PCR 抑制剂),...
An E. coli strain that carries the Phusion DNA Polymerase gene. 产品类别: Phusion® High-Fidelity DNA Polymerases Products 应用: Gibson Assembly®, Long Range PCR, Fast PCR, High-Fidelity PCR, Multiplex PCR, Specialty PCR, Routine PCR, PCR ...
- High yields of PCR products with minimal enzyme amounts 组成成分: ▪ Phusion DNA Polymerase (2 U/µL) ▪ 5X Phusion HF Buffer ▪ 5X Phusion GC Buffer ▪ DMSO ▪ 50 mM MgCl2 solution ▪ 10 mM dNTP Mix Store at -5 to -30°C. ...
肿瘤防治中心(肿瘤医院、肿瘤研究所) 广州市艾毕康生物科技有限责任公司 高保真酶Phusion High-Fidelity DNA Polymerase 2000.0 500u 1.0 2000.0 2024-11-13潜在供应商最新中标 中标单位:广州市艾毕康生物科技有限责任公司共0条查看 6孔细胞培养板-肿瘤防治中心(肿瘤医院、肿瘤研究所) 发布时间:2分钟前 Sigma二甲基...
User Guide: Phusion Hot Start II High-Fidelity DNA Polymerase 常见问题解答 (FAQ) Phusion DNA Polymerases generate blunt end products; therefore, blunt end cloning is recommended. If TA cloning is required, it can be performed by adding A overhangs to the blunt PCR product with e.g. Taq DN...
品名: Phusion High-Fidelity DNA Polymerase 规格:100 units 产品描述: Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polyme...
• High fidelity (52X Taq) • Fast PCR due to short extension times (15-30 s/kb) • Robust reactions, minimal optimization needed • Increased product yields with minimal enzyme amounts • Direct loading on gels 数据: 图1.Phusion Hot Start II DNA Polymerase provides extreme specificit...