The packaging vector pCMVdeltaR8.91, the VSV-G envelope glycoprotein vector pMD.G, and the full-length of PHRF1 in the pLenti6/V5 vector were co-transfected into HEK293 T cells. After 48 h incubation, the viral particles were collected to infect U2OS cells in the presence of polybrene (...
The number of electrons in the outermost shell, or valence electrons, plays a crucial role in determining how the element will interact with others. Elements with a full outer shell are particularly stable, while those with partially filled shells readily form bonds to achieve th...