组蛋白H2AX属于H2A组蛋白家族大众成员,包含一个进化保守区域SQ位于真核细胞的C末端,分子量为14KD。这个保守区的139位丝氨酸能快速磷酸化形成已知的γ-H2AX来指示双链DNA损伤和凋亡。DNA损伤后达到最大磷酸化反应速度一半仅需要1-3分钟,每个双链的断裂区会有成百上千的γ-
Phosphorylated histone H2AX in spheroids, tumors, and tissues of mice exposed to etoposide and 3-amino-1,2,4-benzotriazine-1,3-dioxide. Cancer Res 2004;64:5363-9.Olive PL,Banath JP,Sinnott LT.phosphorylated histone H2AX in spheroids,tumors,and tissues of mice exposed to etoposide and 3-...
Immunocytochemical Detection of Phosphorylated Histone H2AX (cH2AX), Activated ATM and Cell Labeling with EdU问题补充:匿名 2013-05-23 12:21:38 免疫组化检测磷酸化组蛋白H2AX的( cH2AX ) ,激活ATM和细胞标记用EdU 匿名 2013-05-23 12:23:18 immunocytochemical检测磷酸化histone H2ax(CH2AX),激活自...
RAG-2 deficiency results in fewer phosphorylated histone H2AX foci, but increased retinal ganglion cell death and altered axonal growthDevelopmental neurogenesisNeuronal developmentDNA double-strand breaks (DSBs), selectively visualized as 纬-H2AXfoci, occur during the development of the central nervous ...
lated at Serine 139 Histone H2AX Phosphorylated at Serine 139Histone H2AX Phosphorylated at Serine 139doi:10.1007/978-3-540-47648-1_2756γH2AXSpringer Berlin Heidelberg
The γH2A foci, which are established by phosphorylation of histone H2A (H2AX in mammalians) flanking DNA damaged sites, are usually considered as a biomarker during the DNA damage response (39., 40., 41.). Formation of the foci is likely mediated by special scaffolding proteins. Binding ...
Due to its high specificity, the phosphorylated H2AX variant histone assay associated with pATM (Ser1981) combined with flow cytometry was herein used to demonstrate the impact of malnutrition on the DNA damage response. Flow cytometry data indicated that splenic T and B lymphocytes from rats with...
MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. CREB: a Ca(2+)-regulated transcription factor phosphorylated by calmodulin-dependent kinases Fujiwara, H. et al. -Synuclein is phosphorylated in synucleinopathy lesions. Nature Cell Biol. 4, ...
2007. Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast. EMBO Rep 8:851-857.Hammet, A.; Magill, C.; Heierhorst, J.; Jackson, S.P. Rad9 BRCT domain interaction with phosphorylated H2AX regulates the G1 checkpoint in budding yeast. EMBO Rep...
expressingS1219Amutant53BP1wasestablished.IR-inducedfociformationofMDC1andcH2AX,DNAdamagesignalingmolecules,wasreducedinthiscellline,implyingthatS1219phosphorylationisrequiredforrecruitmentofthesemoleculestoDNAdamagesites.Furthermore,overexpressionofthemutantproteinimpededIR-inducedG2arrest.Inconclusion,wehaveshownthatS...