In contrast to the P-enolpyruvate synthetase reaction, the energetics of the pyruvate, phosphate dikinase reaction, favor the formation of pyruvate from P-enolpyruvate rather than P-enolpyruvate synthesis. Because pyruvate kinase has not been detected in Entarnoeba histolyticu and in Bacteroides ...
In addition to the normal carboxylation reaction, phosphoenolpyruvate carboxylase from Zea mays catalyzes a HCO3(-)-dependent hydrolysis of phosphoenolpyruvate to pyruvate and Pi. Two independent methods were used to establish this reaction. First, the formation of pyruvate was coupled to lactate dehydrog...
Phosphoenolpyruvate phosphomutase (PEPPM) catalyzes the C-P bond forming reaction by intramolecular rearrangement of phosphoenolpyruvate to phosphonopyruvate, and shows stronger activity in catalyzing the reverse reaction than the forward reaction. By exploiting the activity of PEPPM to catalyze the reverse...
The active form has a molecular mass of 690+/-20 kDa and is assumed to be octomeric, while approximately 30% of the protein is purified as a large ( approximately 1.6 MDa) complex that is not active. The apparent K(m) values and catalytic efficiencies for the substrates pyruvate and ...
Carboxytransphosphorylase catalyzes the conversion of phosphoenolpyruvate, orthophosphate, and CO2 to oxaloacetate and inorganic pyrophosphate (oxaloacetate reaction), and when CO2 is excluded, the irreversible conversion of phosphoenolpyruvate and phosphate to pyruvate and pyrophosphate (pyruvate reaction). The ...
PEPCK catalyses the reaction from oxaloacetate to make phosphoenol pyruvate and carbon dioxide, part of the gluconeogenesis pathway. Parasite PEPCK catalyses the reverse reaction to form oxaloacetate, the precursor of malate, which is m... AK Verma,A Swargiary,SB Prasad,... 被引量: 4发表: ...
The system catalyzes the transfer of phosphate from phosphoenol pyruvate (PEP) to a variety of sugars and sugar derivatives, and requires three protein fractions, enzymes I and II, and a heatstable protein, called HPr. The latter acts as a "phosphate carrier" in the overall reaction; in ...
In E. coli, PEP also participates as substrate in the reaction catalyzed by the pyruvate kinase (Pyk) isozymes (PykA and PykF), yielding PYR and ATP[5]. Furthermore, PEP connects the EMP pathway and the TCA cycle via an anaplerotic reaction catalyzed by PEP carboxylase (Ppc) that ...
hepatica is controlled by the concentration of reactants and the ambient pH, that the accumulation of GTP is a sensitive mechanism for inhibiting the carboxylation reaction and that PEPCK activity in the cytosol is likely to be favoured over that of pyruvate kinase except when pH is high and ...
The first obligatory step in the biosynthesis of phosphonates is the formation of a carbon-phosphorus bond by converting phosphoenolpyruvate (PEP) to phosphonopyruvate (P-pyr), a reaction that is catalyzed by PEP mutase. The PEP mutase functions as a tetramer and requires magnesium ions (Mg2+)...