The enzyme phosphoenolpyruvate synthetase from Escherichia coli catalyzes the over-all reaction: ATP + pyruvate phosphoenolpyruvate + AMP + Pi. Previous investigations had established the formation of a phospho
Phosphoenolpyruvate phosphomutase (PEPPM) catalyzes the C-P bond forming reaction by intramolecular rearrangement of phosphoenolpyruvate to phosphonopyruvate, and shows stronger activity in catalyzing the reverse reaction than the forward reaction. By exploiting the activity of PEPPM to catalyze the reverse...
The system catalyzes the transfer of phosphate from phosphoenol pyruvate (PEP) to a variety of sugars and sugar derivatives, and requires three protein fractions, enzymes I and II, and a heatstable protein, called HPr. The latter acts as a "phosphate carrier" in the overall reaction; in ...
Pyruvate is converted by pyruvate oxidase in the presence of phosphate and oxygen into acetyl phosphate, carbon dioxide, and hydrogen peroxide. The resulting hydrogen peroxide is then detected with a highly specific fluorometric probe. Horseradish peroxidase catalyzes the reaction between the probe and ...
In addition to the normal carboxylation reaction, phosphoenolpyruvate carboxylase from Zea mays catalyzes a HCO3(-)-dependent hydrolysis of phosphoenolpyruvate to pyruvate and Pi. Two independent methods were used to establish this reaction. First, the formation of pyruvate was coupled to lactate dehydrog...
To determine whether other downstream metabolites compensate for the absence of PEPC, we introduced 1 mM of various metabolites—OAA, citrate, aspartate, pyruvate, glycerol, and malate—into the culture medium. The infection rates of the ∆BgPEPC, BgPEPC-3 × Flag, and WT strains were ...
PEPCK catalyses the reaction from oxaloacetate to make phosphoenol pyruvate and carbon dioxide, part of the gluconeogenesis pathway. Parasite PEPCK catalyses the reverse reaction to form oxaloacetate, the precursor of malate, which is m... AK Verma,A Swargiary,SB Prasad,... 被引量: 4发表: ...
To measure glucose output, cells were seeded in six-well plate. The medium was replaced with 1 ml of glucose-free DMEM supplemented with 2 mM sodium pyruvate and 20 mM sodium lactate. After 3 h incubation, medium was collected and the glucose concentration was measured with Glucose ...
enolpyruvatecarboxylase (PEP C:E .C .4 .1.1_3 11 and pyruvate kinase (PK ;E .C .2.7.1 -4 0) plays a prominent ro le in part iti oning o il s eed carb oh v dr at es to w ard p last idic fatty aci d bio synthesis v ersus the mit ochon drial pro ductio n of...
First, a co-factor (Mg 2 +) binds to the enzyme followed by the substrate (PEP) and finally HCO3−. This is followed by a transfer of a phosphate group from PEP to HCO3− in order to form carboxyphosphate and enolate of pyruvate. At this point, a conformational change is re...