Non-treated HT-29 and TNF alpha (HZ-1014) treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28252-1-AP (Phospho-RIPK1 (Ser166) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loadin...