Olsen and Truog testA water‐extraction procedure which is specific for a pool of P defined by a sorption model based on the Langmuir equation was evaluated in a glasshouse experiment using perennial ryegrass grown in three contrasting soils. For each of three harvests, water‐extractable P ...
According to Kolarovic and Fournier (1986), a 20-ml Pyrex test tube fitted with a polytetrafluoroethylene-line screw cap, containing 200 μl rat heart microsomal suspension (3.6 mg protein) and 200 μl distilled water, was placed in a 50°C water-bath sonicator (100 W). As soon as ...
Preparation of living foraminifera for intracellular phosphate analyses The top 1 cm of sediment was directly wet sieved over a 125 or 250-µm mesh within a time frame of 2 h following core retrieval, using either filtered regional surface water or nitrate- and phosphate-free artificial se...
A noteworthy feature of HUP is that it comprises stacks of lamellae with water molecules bridging adjacent HUP chains. These contain mobile protons that can exchange formetal cationssuch as Ni2+, removing the Ni2+from solution into the HUP lattice via intercalative ion exchange[3]. This approac...
Phosphate dosing is used by water utilities to prevent plumbosolvency in water supply networks. However, there is a lack of knowledge regarding biofilm formation on lead and plastic materials when phosphate concentrations are modified in drinking water systems. In this study, biofilms were grown over...
A plasma membrane PI(4,5)P2 pool was present in the lipid rafts that are enriched in gangliosides (Szymanska et al., 2009; Posse de Chaves and Sipione, 2010). Upon activation of Fcγ receptor IIA in BHK cells, PIP5Kα was recruited to the PI(4,5)P2-rich lipid rafts and co...
It was gently mixed and incubated for one hour at room temperature, followed by the addition of 1% bovine serum albumin (BSA) (Sigma, Missouri, USA) in water (Millipore, USA) to stabilize the mixture. The excess unconjugated antibody was removed and the remaining unbound sites on the ...
2). While the role of glycolysis is the generation of ATP and pyruvate, the PPP is mainly involved in maintaining a pool of cellular NADPH, which may serve as a hydrogen donor in reductive biosynthesis and in the defence against oxidative stress. The PPP also serves to convert G6P to ...
The polar phase was separated by centrifugation, lyophilized and resuspended in water for analysis. Quantitative PCR of PfDXS and PfDXR transcripts Infected erythrocytes were lysed by treatment with 0.1% saponin. Parasite pellets were stored at −80 °C until extraction. Total RNA was isolated ...
The reduction was also evident in each of 100 nm steps from the border of the active zone, indicating that the number of SVs is reduced not only in the reserve pool, but also in the pool proximal to the active zone. Unexpectedly, the number of vesicles asso- ciated with the ...