Protease and phosphatase inhibitor cocktails, tablets, and capsules are ideal for the protection of proteins during extraction or lysate preparation from primary cells, cultured mammalian cells, animal tissues, plant tissues, yeast, or bacterial cells. Individual protea...
The 100X cocktails, available in regular and EDTA-free formulations and several package formats, are refrigerator-stable liquids that are very easy to use for nearly any sample or experimental scale. Highlights: Complete protection all-in-one cocktail contains both protease and phosphatase inhibitors...
The Halt Protease and Phosphatase Inhibitor Cocktails are offered as 100X concentrates in single-use or multiple-use formats. The metalloprotease inhibitor, EDTA, is provided in separate vials.Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support ...
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail (100X) provides the convenience of a single solution with full protein sample protection for cell and tissue lysates. Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extrac...
Figure 1:Using a general protease activity assay and pancreas extract, our Turbocharged Cocktails were tested against Competitor’s commercially available tablets under the same conditions. The results were based on protease activity in the absence of any inhibitor cocktails (Test 1); compared to prot...
Numerous traditional phosphatase and protease inhibitor cocktails can cause issues during analysis by mass spectrometry necessitating caution with their use. We have derived an MS friendly cocktail which has been designed for maximal protease and phosphatase inhibition containing no irreversible covalent ...
Features of Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X):• Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors• Multiple package sizes—choose from four convenient packages sizes to match nearly any scale• Convenient —the 100X liquid ...
After 2 days, cells were harvested in lysis buffer (6 m guanidine·HCl, 100 mm sodium phosphate buffer-pH 8.0, 5 mm imidazole, 5 mm N-ethylmaleimide, and protease and phosphatase inhibitor cocktails). The lysates were then sonicated to shear the genomic DNA, and centrifuged at 14,000 × ...
For western blotting, cells were lysed in RIPA buffer (50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.5% v/v sodium deoxycholate, 1% v/v Nonidet P-40, 0.1% SDS) supplemented with protease and phosphatase inhibitor cocktails. Lysates were resolved by SDS-PAGE and transferred to nitrocellulose ...
For protein extraction, isolated ileal epithelium was subjected to ice-cold RIPA Lysis and Extraction Buffer (Thermo Fisher Scientific) containing proteinase inhibitor cocktails (Millipore) and homogenized by beads-beating using Vortex-Genie 2 mixer (Scientific Industries, Inc.) for 30 min at 4˚...