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Scale bars: 100 μm (upper panels) or 25 μm (lower panels). (c,d) The mRNA (c) and protein (d) expression levels of angiogenic factors in C2C12 cells treated with salidroside were analyzed by using quantitative RT-PCR (*p < 0.05, **p < 0.01, data...
Signaling hypoxia by hypoxia-inducible factor protein hydroxylases: a historical overview and future perspectives. Hypoxia 2014; 2: 197–213. 8. Kaelin WG Jr., Ratcliffe PJ. Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. Mol Cell 2008; 30: 393–402. 9. ...
4A–C). This was associated with increased gene and protein expression of the lipogenic enzymes ACC and FAS (Fig. 4D–G) compared to WT mice. Of note, similar increase in lipogenic gene expression were also found in PDH1−/− mice on regular chow diet when compared with WT mice (...
Black horizontal lines represent the mean, and the error bars represent ±S.D. from the number of plants counted for each genotype. n = 15 per line. c Western blotting result showing the accumulation levels of AIPP3 proteins in different transgenes. Ponceaus staining serves as protein loading...
(C) MePHD1 interacts with the Seg2 by DPI-ELISA assay. Seg2: Double-strand biotinylated Seg2 DNA probe + purified MePHD1 fusion protein; CK1: Double-strand biotinylated Seg2 DNA probe + tagged protein ProS2; CK2: Purified water + purified MePHD1 fusion protein. Error bars represent the...
MePHD1 protein could repress the promoter activity of MeAGPS1a gene by a dual-luciferase assay; which indicated that MePHD1 is a negative regulator for the transcript level of MeAGPS1a gene. Keywords: cassava; plant homeodomain transcription factor; ADP-glucose pyrophosphorylase; starch biosynthesis...
The scale bars represent 100 nm, Figure S5. HIF-1α protein levels in HeLa cells stabilized by free IOX2 and liposomal IOX2 at (a) 6 h and (b) 24 h. The time-dependent results showed the decreased drug effects in both treatment groups at 24 h, suggesting that the peak of IOX2 ...
Green color is GFP protein signal, and blue color represents DAPI stained for nucleus. Bars = 50 μm. 3. Discussion PHD-finger proteins have been identified in different plant species as involved in various biological processes, including the regulation of chromatin structure and transcription, ...