39 In short, cell lysates were dissolved in 12% SDS-PAGE by immunoblotting with a LC3 antibody. An ECL detection system (Thermo Fisher Scientific, Schwerte, Germany) was used in this process. Immunofluorescence assay Primary antibodies, EEA1, P62, Caveolin, Cdc42, Clathrin, LC3, Flotillin, ...
After SDS-PAGE, the membranes blotted with proteins were blocked with 4% BlockAce solution (Dainippon Pharmaceutical, Osaka, Japan) for 1 h and then incubated with antibodies against the following proteins: BIG3 (1:1,000); PHB2 (1:1,000, Abcam, Cambridge, UK); ERα (SP-1, 1:500...
Stomatin-like 2 CCK8: Cell counting kit 8 PMSF: Phenylmethylsulfonyl fluoride PBS: Phosphate Buffered Saline SDS-PAGE: Sodium dodecyl sulfatepolyacrylamide gel electrophoresis PVDF: Poly (vinylidene fluoride) FFPE: Formalin fixed paraffin-embedded HRP: Horseradish Peroxidase SD: Synthetic dr...
The inhibitory effect of various chemical reagents, diazo-dl-norleucine methyl ester (DAN),N-p-tosyl-l-lysinechloromethyl ketone (TLCK),N-ethylmaleimide (NEM),N-acetylimidazole (NAI), iodoacetate (IA), dithiothreitol (DTT), phenylmethylsulphonyl fluoride (PMSF), EDTA, SDS and urea, on enzyme...
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Immunoprecipitated protein complexes were washed three times with lysis buffer and separated using SDS–PAGE. Immunoblot analyses were performed as described above. Identification of the PHB2-binding regions in BIG3 protein. To determine the PHB2-binding region in BIG3, we cloned five different ...
Table 2.Central composite design along with experimental and predicted values of dependent variable for PHB production. 2.11. Statistical Analysis Each experiment was performed in triplicate, and the results were expressed as means ± SDs. GraphPad Prism 5.0 (GraphPad Software, Inc., San Diego, CA...
polymers Article Characterization and Evaluation of Controlled Antimicrobial Release from Petrochemical (PU) and Biodegradable (PHB) Packaging Alexey Iordanskii 1,* ID , Anna Zhulkina 1, Anatoliy Olkhov 1,2 ID , Sergey Fomin 3, Andrey Burkov 3 and Mikhail Stilman 4 1 Semenov Institute of ...
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