intrinsically disordered proteinsRNA binding proteinsheat soluble proteinsphase separated granulesProteasomal degradation is the main route of regulated proteostasis. The 20S proteasome is the core particle (CP) responsible for the catalytic activity of all proteasome complexes. Structural constraints mean that...
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et al. RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether. Cell 179, 147–164 (2019). CAS PubMed PubMed Central Google Scholar Padrón, A., Iwasaki, S. & Ingolia, N. T. Proximity RNA labeling by APEX-Seq reveals the organization ...
Although membrane-less organelles were known for a long time, it wasn't until the pivotal research on P granules inCaenorhabditis elegansthat it was understood that these membrane-less organelles largely form through a process known as phase separation (Brangwynne et al.2009). Historically, these ...
C-TERMINAL REGION 8 (ECT8) act together as a key checkpoint for negative feedback regulation of abscisic acid (ABA) signalling by sequestering the m6A-modified ABA receptor gene PYRABACTIN RESISTANCE 1-LIKE 7 (PYL7) via phase-separated ECT8 condensates in stress granules in response to ABA....
P granules are non-membrane-bound RNA-protein compartments that are involved ingermlinedevelopment inC. elegans. They are liquids that condense at one end of the embryo by localized phase separation, driven by gradients of polarity proteins such as the mRNA-binding protein MEX-5. To probe how ...
1A). This agrees with cellular component GSEA terms, which are also associated with the chromosomal regions, ribonucleoprotein granules, and spliceosomal complexes (Fig. 1B). Download: Download high-res image (538KB) Download: Download full-size image Fig. 1. GSEA of differentially expressed ...
Uncovering the driving forces behind partitioning of proteins into phase-separated granulesdoi:10.1016/j.bpj.2023.11.2987Gregory L. DignonBiophysical Journal
2.2.1. Stress Granules Both stress granules and processing bodies (P bodies) are composed of RNA and protein molecules that drive the phase separation of these membraneless organelles. Stress granule formation is exclusively induced by stress signals imposed on the cells, while P bodies can be con...
RNA binding intrinsically disordered proteins (IDPs), which form phase鈥搒eparated granules within the cell, are a large subset of these substrates. These proteins in addition to harboring an RNA binding domain (RBD) are enriched with prion like domains (PrLD) and low complexity domains (LCD)...